检测CV2（CRMP5）抗体时的陷阱。
Pitfalls in the detection of CV2 (CRMP5) antibodies.
作者信息
Sabater Lidia, Saiz Albert, Dalmau Josep, Graus Francesc
机构信息
Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Service of Neurology, Hospital Clinic, University of Barcelona, Spain.
出版信息
J Neuroimmunol. 2016 Jan 15;290:80-3. doi: 10.1016/j.jneuroim.2015.11.009. Epub 2015 Dec 1.
CV2 antibodies (CV2-ab) associate with paraneoplastic neurological syndromes (PNS) and small-cell lung cancer. This study was designed to assess the sensitivity of two widely used anti-CV2 commercial kits. Fifty three sera with CV2-ab identified by immunohistochemistry on paraformaldehyde-perfused rat brain were tested with two commercial immunoblot kits (Euroimmun AG, and Ravo Diagnostika) and 4 (7.5%) of them were negative with the commercial kits. The 4 samples were positive by immunofluorescence on HEK293 cells transfected with CRMP5 and immunoblot of these cells lysate. A few CV2-ab-positive sera may be missed by commercial immunoblots. Negative samples from patients with high suspicion for PNS should be tested by alternative methods.
抗CV2抗体（CV2-ab）与副肿瘤性神经综合征（PNS）及小细胞肺癌相关。本研究旨在评估两种广泛使用的抗CV2商业试剂盒的敏感性。对53份经免疫组化在多聚甲醛灌注大鼠脑上鉴定为CV2-ab阳性的血清，使用两种商业免疫印迹试剂盒（Euroimmun AG和Ravo Diagnostika）进行检测，其中4份（7.5%）样本用商业试剂盒检测为阴性。这4个样本在用CRMP5转染的HEK293细胞上进行免疫荧光检测以及对这些细胞裂解物进行免疫印迹时呈阳性。一些CV2-ab阳性血清可能会被商业免疫印迹法漏检。对高度怀疑PNS的患者的阴性样本应采用其他方法进行检测。
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