Mohammadi Nabiallah, Adib Minoo, Alsahebfosoul Fereshteh, Kazemi Mohammad, Etemadifar Masoud
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
J Neuroimmunol. 2016 Jan 15;290:115-8. doi: 10.1016/j.jneuroim.2015.11.019. Epub 2015 Nov 27.
Human Leukocyte Antigen G (HLA-G) gene polymorphism and expression rate have recently been suggested to have a potential role in susceptibility to Multiple Sclerosis (MS), a chronic inflammatory demyelinating and neurodegenerative disease of the central nervous system with unknown etiology. The aim of this study was to investigate the association of the frequency of HLA-G gene 14 bp insertion/deletion polymorphism and its plasma level with MS susceptibility. In this study, the HLA-G gene from 212 patients and 210 healthy individuals was amplified using real time PCR and screened for the 14 bp insertion/deletion polymorphism. In addition, HLA-G plasma levels of the patients were measured and compared to normal controls by ELISA method. Our results revealed that 14 bp insertion in HLA-G could result in lower plasma HLA-G level of the subjects, regardless of their health status and vice versa. Additionally, significant correlation of HLA-G genotype and its plasma level with MS susceptibility was observed. In conclusion, not only HLA-G 14 bp insertion/deletion polymorphism could be associated with expression rate of the HLA-G gene and its plasma level, but also could be considered as a risk factor for susceptibility to MS in our study population.
人类白细胞抗原G（HLA - G）基因多态性和表达率最近被认为在多发性硬化症（MS）易感性中具有潜在作用，MS是一种病因不明的中枢神经系统慢性炎性脱髓鞘和神经退行性疾病。本研究的目的是调查HLA - G基因14 bp插入/缺失多态性频率及其血浆水平与MS易感性的关联。在本研究中，使用实时PCR扩增了212例患者和210例健康个体的HLA - G基因，并筛选14 bp插入/缺失多态性。此外，通过ELISA法测量患者的HLA - G血浆水平并与正常对照进行比较。我们的结果显示，无论健康状况如何，HLA - G基因中的14 bp插入均可导致受试者血浆HLA - G水平降低，反之亦然。此外，观察到HLA - G基因型及其血浆水平与MS易感性存在显著相关性。总之，在我们的研究人群中，不仅HLA - G 14 bp插入/缺失多态性可能与HLA - G基因的表达率及其血浆水平相关，而且可被视为MS易感性的一个危险因素。
