Jaklenec Ana, Anselmo Aaron C, Hong Jinkee, Vegas Arturo J, Kozminsky Molly, Langer Robert, Hammond Paula T, Anderson Daniel G
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology , 500 Main Street, Cambridge, Massachusetts 02139, United States.
Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
ACS Appl Mater Interfaces. 2016 Jan 27;8(3):2255-61. doi: 10.1021/acsami.5b11081. Epub 2016 Jan 12.
A high-throughput approach which automates the synthesis of polyelectrolyte-based layer-by-layer films (HT-LbL) to facilitate rapid film generation, systematic film characterization, and rational investigations into their interactions with cells is described. Key parameters, such as polyelectrolyte adsorption time and polyelectrolyte deposition pH, were used to modulate LbL film growth to create LbL films of distinct thicknesses using the widely utilized polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA). We highlight how HT-LbL can be used to rapidly characterize film-forming parameters and robustly create linearly growing films of various molecular architectures. Film thickness and growth rates of HT-LbL films were shown to increase as a function of adsorption time. Subsequently, we investigated the role that polyelectrolyte solution pH (ranging from 2.5 to 9) has in forming molecularly distinct films of weak polyelectrolytes and report the effect this has on modulating cell attachment and spreading. Films synthesized at PAA-pH of 5.5 and PAH-pH 2.5-5.5 exhibited the highest cellular attachment. These results indicate that HT-LbL is a robust method that can shift the paradigm regarding the use of LbL in biomedical applications as it provides a rapid method to synthesize, characterize, and screen the interactions between molecularly distinct LbL films and cells.
本文描述了一种高通量方法，该方法可自动合成基于聚电解质的层层薄膜（HT-LbL），以促进快速成膜、系统的薄膜表征以及对其与细胞相互作用的合理研究。使用诸如聚电解质吸附时间和聚电解质沉积pH值等关键参数来调节LbL薄膜的生长，从而使用广泛使用的聚电解质聚（烯丙胺盐酸盐）（PAH）和聚（丙烯酸）（PAA）创建不同厚度的LbL薄膜。我们强调了HT-LbL如何可用于快速表征成膜参数，并稳健地创建各种分子结构的线性生长薄膜。结果表明，HT-LbL薄膜的厚度和生长速率随吸附时间的增加而增加。随后，我们研究了聚电解质溶液pH值（范围为2.5至9）在形成弱聚电解质分子结构不同的薄膜中的作用，并报告了其对调节细胞附着和铺展的影响。在PAA-pH为5.5和PAH-pH为2.5 - 5.5条件下合成的薄膜表现出最高的细胞附着率。这些结果表明，HT-LbL是一种强大的方法，它可以改变LbL在生物医学应用中的使用模式，因为它提供了一种快速合成、表征和筛选分子结构不同的LbL薄膜与细胞之间相互作用的方法。
