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本文引用的文献

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Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials.视网膜祖细胞在可移植生物材料的细胞外基质底物上的集体黏附和位移。
J Tissue Eng. 2018 Jan 9;9:2041731417751286. doi: 10.1177/2041731417751286. eCollection 2018 Jan-Dec.
2
High-throughput approaches for screening and analysis of cell behaviors.高通量方法在细胞行为筛选和分析中的应用。
Biomaterials. 2018 Jan;153:85-101. doi: 10.1016/j.biomaterials.2017.06.022. Epub 2017 Jun 21.
3
Solvent-Assisted Micromolding of Biohybrid Hydrogels to Maintain Human Hematopoietic Stem and Progenitor Cells Ex Vivo.溶剂辅助微成型法制备生物杂化水凝胶以维持人造血干/祖细胞的体外扩增
Adv Mater. 2017 Nov;29(42). doi: 10.1002/adma.201703489. Epub 2017 Sep 28.
4
Healing of a Large Long-Bone Defect through Serum-Free In Vitro Priming of Human Periosteum-Derived Cells.通过无血清体外预刺激人骨膜来源细胞修复大段长骨缺损
Stem Cell Reports. 2017 Mar 14;8(3):758-772. doi: 10.1016/j.stemcr.2017.01.005. Epub 2017 Feb 9.
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High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation.粘着斑依赖性机械敏感干细胞分化的高内涵图像分析
Integr Biol (Camb). 2016 Oct 10;8(10):1049-1058. doi: 10.1039/c6ib00076b.
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Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers.基于天然源聚电解质多层的仿生细胞外环境。
Small. 2016 Aug;12(32):4308-42. doi: 10.1002/smll.201601355. Epub 2016 Jul 20.
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The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.人骨膜来源细胞通过骨形态发生蛋白和磷酸钙诱导骨骼组织形成的联合机制
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8
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Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.用于人类多能干细胞扩增和多谱系分化的新型聚合物的发现。
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用于高通量筛选细胞行为的细胞培养微孔板中仿生膜的自动构建。

Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors.

机构信息

Grenoble Institute of Technology, Université Grenoble Alpes, 38000, Grenoble, France.

CNRS, LMGP, 3 parvis Louis Néel, 38031, Grenoble, Cedex 01, France.

出版信息

Adv Mater. 2018 Jul;30(27):e1801097. doi: 10.1002/adma.201801097. Epub 2018 May 22.

DOI:10.1002/adma.201801097
PMID:29786885
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6701983/
Abstract

An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film-coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide-functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell-based assays for regenerative medicine and high-throughput drug screening.

摘要

建立了一种使用商用液体处理机器人在细胞培养微孔板中逐层（LbL）组装仿生涂层的自动方法。在每个微孔的底部形成高度均匀的薄膜。LbL 薄膜涂覆的微孔板与常见的细胞测定法兼容，可使用微孔板读数器和自动显微镜。在交联和肽功能化的 LbL 薄膜上筛选细胞黏附，并响应骨形态发生蛋白（2、4、7、9）的递增剂量检测干细胞分化。该方法为 LbL 薄膜在基于细胞的再生医学和高通量药物筛选测定法中的未来应用铺平了道路。