Zhao Xiuqin, Zhang Guilian, Wang Yun, Zhang Fan, Wang Wensheng, Zhang Wenhao, Fu Binying, Xu Jianlong, Li Zhikang
Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Shanghai Landscape Gardening Research Institute, Shanghai 200232, China.
PLoS One. 2015 Dec 29;10(12):e0145646. doi: 10.1371/journal.pone.0145646. eCollection 2015.
A rice introgression line, NIL-SS1, and its recurrent parent, Teqing, were used to investigate the influence of the introgression segment on plant growth. The current research showed NIL-SS1 had an increased flag leaf width, total leaf area, spikelet number per panicle and grain yield, but a decreased photosynthetic rate. The metabolite differences in NIL-SS1 and Teqing at different developmental stages were assessed using gas chromatography-mass spectrometry technology. Significant metabolite differences were observed across the different stages. NIL-SS1 increased the plant leaf nitrogen content, and the greatest differences between NIL-SS1 and Teqing occurred at the booting stage. Compared to Teqing, the metabolic phenotype of NIL-SS1 at the booting stage has closer association with those at the flowering stage. The introgression segment induced more active competition for sugars and organic acids (OAs) from leaves to the growing young spikes, which resulted in more spikelet number per plant (SNP). The results indicated the introgression segment could improve rice grain yield by increasing the SNP and total leaf area per plant, which resulted from the higher plant nitrogen content across growth stages and stronger competition for sugars and OAs of young spikes at the booting stage.
利用一个水稻渐渗系NIL-SS1及其轮回亲本特青，研究渐渗片段对植株生长的影响。当前研究表明，NIL-SS1的剑叶宽度、总叶面积、每穗颖花数和籽粒产量增加，但光合速率降低。采用气相色谱-质谱技术评估了NIL-SS1和特青在不同发育阶段的代谢物差异。在不同阶段观察到显著的代谢物差异。NIL-SS1提高了植株叶片氮含量，NIL-SS1与特青之间的最大差异出现在孕穗期。与特青相比，NIL-SS1在孕穗期的代谢表型与开花期的代谢表型关联更紧密。渐渗片段引发了从叶片到生长中的幼穗对糖类和有机酸（OAs）更活跃的竞争，这导致单株颖花数（SNP）增加。结果表明，渐渗片段可通过增加SNP和单株总叶面积来提高水稻籽粒产量，这是由于整个生长阶段植株氮含量较高以及孕穗期幼穗对糖类和OAs的竞争更强所致。
