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灰小鼠负鼠(Tlacuatzin canescens)的完整线粒体基因组

Complete mitochondrial genome of Tlacuatzin canescens (Grayish Mouse Opossum).

作者信息

Pang Xin

机构信息

a College of Marine Life Sciences , Ocean University of China , Qingdao , PR China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Mar;28(2):283-284. doi: 10.3109/19401736.2015.1118081. Epub 2015 Dec 29.

Abstract

In this study, we report the complete mitochondrial genome of the Grayish Mouse Opossumm, Tlacuatzin canescens. The mitochondrial genome is 16 460 bp in length and has a base composition of A (35.7%), T (31.3%), C (21.3%), and G (11.7%), demonstrating an obvious bias of high AT content (67%). The mitochondrial genome contains a typically conserved structure, encoding 13 protein-coding genes (PCGs), 20 transfer RNA genes (tRNA), 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and a control region (D-loop region). Except for ND6 gene, all other PCGs were located on the H-strand. ND4 gene and ND4L gene were overlapped by 4 bp, and ATP8 gene and ATP6 gene were overlapped by 45 bp. The nucleotide sequence of 13 PCGs of T. canescens and other 30 Didelphimorphia species were used for phylogenetic analysis. The result indicated T. canescens a relative close relationship with species Monodelphis domestica.

摘要

在本研究中,我们报道了灰鼠负鼠(Tlacuatzin canescens)的完整线粒体基因组。该线粒体基因组长度为16460 bp,碱基组成为A(35.7%)、T(31.3%)、C(21.3%)和G(11.7%),显示出明显的高AT含量偏向(67%)。线粒体基因组包含典型的保守结构,编码13个蛋白质编码基因(PCGs)、20个转运RNA基因(tRNA)、2个核糖体RNA基因(12S rRNA和16S rRNA)以及一个控制区(D环区)。除ND6基因外,所有其他PCGs均位于重链上。ND4基因和ND4L基因重叠4 bp,ATP8基因和ATP6基因重叠45 bp。利用灰鼠负鼠的13个PCGs的核苷酸序列和其他30种袋鼬目物种进行系统发育分析。结果表明,灰鼠负鼠与家短尾负鼠(Monodelphis domestica)物种关系相对密切。

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