Wang Tao, Wang Yunfang, Xu Fengqiang, Li Xia, Qu Rui, Song Lin, Tang Yongping, Lin Peilin
Cardiovascular Disease Hospital Affiliated to Qingdao University, Qingdao, Shandong, China.
Qingdao Municipal Hospital, Qingdao, Shandong, China.
Mitochondrial DNA B Resour. 2018 Jun 26;3(2):715-716. doi: 10.1080/23802359.2018.1483763.
In this study, the complete mitochondrial genome of human lung fluke, , was recovered through Illumina sequencing data. This complete mitochondrial genome of is 13,927 bp in length and has a base composition of A (16.6%), T (41.8%), C (13.%), and G (28.4%), demonstrating an obvious bias of high AT content (58.4%). The mitochondrial genome contains a typically conserved structure, encoding 12 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and a control region (D-loop region). All PCGs were located on the H-strand. ND4 gene and ND4L gene were overlapped by 39 bp. The nucleotide sequence of 12 PCGs of and other 10 parasite species were used for phylogenetic analysis. The result indicated a relative close relationship with species (AF219379.2).
在本研究中，通过Illumina测序数据获得了人体肺吸虫的完整线粒体基因组。该人体肺吸虫的完整线粒体基因组长度为13,927 bp，碱基组成为A（16.6%）、T（41.8%）、C（13.%）和G（28.4%），显示出明显的高AT含量偏向（58.4%）。线粒体基因组包含典型的保守结构，编码12个蛋白质编码基因（PCGs）、22个转运RNA基因（tRNA）、2个核糖体RNA基因（12S rRNA和16S rRNA）以及一个控制区（D-loop区）。所有PCGs都位于重链上。ND4基因和ND4L基因重叠39 bp。将该人体肺吸虫的12个PCGs的核苷酸序列与其他10种寄生虫物种的序列用于系统发育分析。结果表明它与物种（AF219379.2）关系相对密切。
