Kim Woo Jin, Kim Byung-Guk, Chang Ki-Hong, Oh Jeong-Hoon
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, The Catholic University of Korea, 180 Wangsan-Ro, Dongdaemun-Gu, Seoul, 130-709, South Korea.
J Otolaryngol Head Neck Surg. 2015 Dec 29;44:58. doi: 10.1186/s40463-015-0111-5.
Bacterial infection, Eustachian tube dysfunction, allergies, and immunologic factors are major causes of otitis media with effusion (OME). However, the exact pathogenesis of OME is still unclear. This study evaluated whether allergy influences bacterial growth in middle ear effusions.
Fifty-four samples were obtained from OME patients 3-10 years of age who underwent ventilation tube insertion and were divided into two groups based on the presence of allergy as determined using the multiple allergosorbent test (MAST). Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis bacterial DNA in the middle ear effusions was analyzed using polymerase chain reaction. Overall detection rates and those for each species were compared between the two groups.
Of the 54 middle ear effusion samples, 38 (70.4 %) contained bacterial DNA and 14 (36.8 %) of these contained DNA from multiple species. S. pneumoniae was detected in 27 samples (50 %), H. influenzae in 17 samples (31.4 %), and M. catarrhalis in 9 samples (16.6 %). There was no significant difference in the bacterial detection rates between the middle ear effusions of the MAST-positive and MAST-negative groups.
The rate of bacteria detection in middle ear effusions did not differ between allergic and non-allergic children.
细菌感染、咽鼓管功能障碍、过敏和免疫因素是分泌性中耳炎（OME）的主要病因。然而，OME的确切发病机制仍不清楚。本研究评估了过敏是否会影响中耳积液中的细菌生长。
从3至10岁接受通气管插入术的OME患者中获取54份样本，并根据多重变应原吸附试验（MAST）确定的过敏情况将其分为两组。使用聚合酶链反应分析中耳积液中的肺炎链球菌、流感嗜血杆菌和卡他莫拉菌的细菌DNA。比较两组的总体检出率及每种细菌的检出率。
在54份中耳积液样本中，38份（70.4%）含有细菌DNA，其中14份（36.8%）含有多种细菌的DNA。27份样本（50%）检测到肺炎链球菌，17份样本（31.4%）检测到流感嗜血杆菌，9份样本（16.6%）检测到卡他莫拉菌。MAST阳性组和MAST阴性组的中耳积液细菌检出率无显著差异。
过敏儿童和非过敏儿童中耳积液中的细菌检出率无差异。
