Rao Vasudev R, Eugenin Eliseo A, Prasad Vinayaka R
Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY, 10461, USA.
Public Health Research Institute (PHRI), Rutgers The State University of New Jersey, Newark, NJ, USA.
Methods Mol Biol. 2016;1354:367-76. doi: 10.1007/978-1-4939-3046-3_25.
Despite the inability of HIV-1 to infect neurons, over half of the HIV-1-infected population in the USA suffers from neurocognitive dysfunction. HIV-infected immune cells in the periphery enter the central nervous system by causing a breach in the blood-brain barrier. The damage to the neurons is mediated by viral and host toxic products released by activated and infected immune and glial cells. To evaluate the toxicity of any viral isolate, viral protein, or host inflammatory protein, we describe a protocol to assess the neuronal apoptosis and synaptic compromise in primary cultures of human neurons and astrocytes.
尽管HIV-1无法感染神经元，但美国超过一半的HIV-1感染人群患有神经认知功能障碍。外周被HIV感染的免疫细胞通过破坏血脑屏障进入中枢神经系统。神经元的损伤是由活化并被感染的免疫细胞和神经胶质细胞释放的病毒及宿主毒性产物介导的。为了评估任何病毒分离株、病毒蛋白或宿主炎症蛋白的毒性，我们描述了一种方案，用于评估原代培养的人神经元和星形胶质细胞中的神经元凋亡和突触损伤。
