Keita Mamadou Bhoye, Padhmanabhan Roshan, Robert Catherine, Delaporte Eric, Raoult Didier, Fournier Pierre-Edouard, Bittar Fadi
Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.
IRD, University Montpellier 1, Montpellier, France.
Microb Ecol. 2016 May;71(4):990-8. doi: 10.1007/s00248-015-0722-4. Epub 2015 Dec 29.
Strain G4(T) was isolated from the stool sample of a wild gorilla (Gorilla gorilla gorilla) from Cameroon. It is a facultative anaerobic, Gram-negative, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.48% with Paenibacillus typhae, the phylogenetically closest species with standing nomenclature. Moreover, the strain G4(T) presents some phenotypic differences when compared to other Paenibacillus species and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here, we describe the characteristics of this organism, complete genome sequence, and annotation. The 6,933,847 bp size genome (1 chromosome but no plasmid) contains 5972 protein-coding genes and 54 RNAs genes, including 44 tRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G4(T) against the closest Paenibacillus genomes range between 19.7 and 22.1, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Paenibacillus camerounensis sp. nov. that contains the strain G4(T).
菌株G4(T)是从喀麦隆一只野生大猩猩（西部低地大猩猩）的粪便样本中分离出来的。它是一种兼性厌氧、革兰氏阴性、杆状细菌。该菌株与系统发育关系最密切的已命名物种嗜热芽孢杆菌的16S rRNA核苷酸序列相似度为97.48%。此外，与其他芽孢杆菌属物种相比，菌株G4(T)表现出一些表型差异，并且基质辅助激光解吸电离飞行时间质谱得分较低，无法进行鉴定。因此，该菌株很可能代表一个新物种。在此，我们描述了这种生物体的特征、完整的基因组序列和注释。该基因组大小为6,933,847 bp（1条染色体，无质粒），包含5972个蛋白质编码基因和54个RNA基因，其中包括44个tRNA基因。此外，菌株G4(T)的基因组与最接近的芽孢杆菌属基因组的数字DNA-DNA杂交值在19.7至22.1之间，再次证实了其作为新物种的新地位。基于这些由表型和基因组分析组成的多相数据，我们提议创建包含菌株G4(T)的喀麦隆芽孢杆菌新种。
