Dehghani Soltani Samereh, Babaee Abdolreza, Shojaei Mohammad, Salehinejad Parvin, Seyedi Fatemeh, JalalKamali Mahshid, Nematollahi-Mahani Seyed Noureddin
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Afzal Research Institute, Kerman, Iran.
Lasers Med Sci. 2016 Feb;31(2):255-61. doi: 10.1007/s10103-015-1846-y. Epub 2015 Dec 29.
Light-emitting diodes (LED) have recently been introduced as a potential factor for proliferation of various cell types in vitro. Nowadays, stem cells are widely used in regenerative medicine. Human umbilical cord matrix-derived mesenchymal (hUCM) cells can be more easily isolated and cultured than adult mesenchymal stem cells. The aim of this study was to evaluate the effect of red and green lights produced by LED on the proliferation of hUCM cells. hUCM cells were isolated from the umbilical cord, and light irradiation was applied at radiation energies of 0.318, 0.636, 0.954, 1.59, 3.18, 6.36, 9.54, and 12.72 J/cm(2). Irradiation of the hUCM cells shows a significant (p < 0.05) increase in cell number as compared to controls after 40 h. In addition, cell proliferation on days 7, 14, and 21 in irradiated groups were significantly (p < 0.001) higher than that in the non-irradiated groups. The present study clearly demonstrates the ability of red and green lights irradiation to promote proliferation of hUCM cells in vitro. The energy applied to the cells through LED irradiation is an effective factor with paradoxical alterations. Green light inserted a much profound effect at special dosages than red light.
发光二极管（LED）最近被引入，作为体外多种细胞类型增殖的一个潜在因素。如今，干细胞在再生医学中被广泛应用。人脐带基质间充质（hUCM）细胞比成人间充质干细胞更容易分离和培养。本研究的目的是评估LED产生的红光和绿光对hUCM细胞增殖的影响。从脐带中分离出hUCM细胞，并以0.318、0.636、0.954、1.59、3.18、6.36、9.54和12.72 J/cm²的辐射能量进行光照射。与对照组相比，照射hUCM细胞40小时后细胞数量显著增加（p < 0.05）。此外，照射组在第7、14和21天的细胞增殖显著高于未照射组（p < 0.001）。本研究清楚地证明了红光和绿光照射在体外促进hUCM细胞增殖的能力。通过LED照射施加到细胞上的能量是一个具有矛盾变化的有效因素。在特定剂量下，绿光比红光产生的影响更为深远。
