一种潜在的弓形虫DNA探针的分离
Isolation of a potential DNA probe for Toxoplasma gondii.
作者信息
Savva D
机构信息
Department of Biochemistry and Physiology, University of Reading, Great Britain.
出版信息
Microbios. 1989;58(236-237):165-72.
A genomic DNA library for Toxoplasma gondii has been constructed in plasmid pUC8. Some recombinant plasmids (pJMBG1-pJMBG55) were analysed by restriction endonuclease mapping and by hybridization to DNA isolated from T. gondii and from human tissue culture cells. A number of recombinant plasmids, or DNA fragments derived from these, hybridizing only to Toxoplasma DNA have been identified. These are currently being characterized for the development of diagnostic DNA probes for Toxoplasma.
已在质粒pUC8中构建了刚地弓形虫的基因组DNA文库。通过限制性内切酶图谱分析以及与从刚地弓形虫和人组织培养细胞中分离的DNA进行杂交，对一些重组质粒（pJMBG1 - pJMBG55）进行了分析。已鉴定出一些仅与弓形虫DNA杂交的重组质粒或从这些质粒衍生的DNA片段。目前正在对这些进行表征，以开发用于弓形虫的诊断性DNA探针。
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