Boakye Lorraine A, Ross Keir A, Pinski John M, Smyth Niall A, Haleem Amgad M, Hannon Charles P, Fortier Lisa A, Kennedy John G
Lorraine A Boakye, Keir A Ross, John M Pinski, Niall A Smyth, Amgad M Haleem, Charles P Hannon, John G Kennedy, Department of Orthopaedic Surgery, Hospital for Special Surgery, New York, NY 10021, United States.
World J Orthop. 2015 Dec 18;6(11):961-9. doi: 10.5312/wjo.v6.i11.961.
To explore the effect of platelet-rich plasma on protein expression patterns of transforming growth factor-beta1 (TGF-β1) in cartilage following autologous osteochondral transplantation (AOT) in a rabbit knee cartilage defect model.
Twelve New Zealand white rabbits received bilateral AOT. In each rabbit, one knee was randomized to receive an autologous platelet rich plasma (PRP) injection and the contralateral knee received saline injection. Rabbits were euthanized at 3, 6 and 12 wk post-operatively. Articular cartilage sections were stained with TGF-β1 antibody. Histological regions of interest (ROI) (left, right and center of the autologous grafts interfaces) were evaluated using MetaMorph. Percentage of chondrocytes positive for TGF-β1 was then assessed.
Percentage of chondrocytes positive for TGF-β1 was higher in PRP treated knees for selected ROIs (left; P = 0.03, center; P = 0.05) compared to control and was also higher in the PRP group at each post-operative time point (P = 6.6 × 10(-4), 3.1 × 10(-4) and 7.3 × 10(-3) for 3, 6 and 12 wk, respectively). TGF-β1 expression was higher in chondrocytes of PRP-treated knees (36% ± 29% vs 15% ± 18%) (P = 1.8 × 10(-6)) overall for each post-operative time point and ROI.
Articular cartilage of rabbits treated with AOT and PRP exhibit increased TGF-β1 expression compared to those treated with AOT and saline. Our findings suggest that adjunctive PRP may increase TGF-β1 expression, which may play a role in the chondrogenic effect of PRP in vivo.
在兔膝关节软骨缺损模型中，探讨富血小板血浆对自体骨软骨移植（AOT）后软骨中转化生长因子-β1（TGF-β1）蛋白表达模式的影响。
12只新西兰白兔接受双侧AOT。每只兔子的一侧膝关节随机接受自体富血小板血浆（PRP）注射，对侧膝关节接受生理盐水注射。术后3、6和12周对兔子实施安乐死。用TGF-β1抗体对关节软骨切片进行染色。使用MetaMorph评估感兴趣的组织学区域（ROI）（自体移植物界面的左侧、右侧和中心）。然后评估TGF-β1阳性软骨细胞的百分比。
与对照组相比，PRP治疗的膝关节中选定ROI（左侧；P = 0.03，中心；P = 0.05）的TGF-β1阳性软骨细胞百分比更高，并且在每个术后时间点PRP组也更高（3、6和12周时分别为P = 6.6×10⁻⁴、3.1×10⁻⁴和7.3×10⁻³）。在每个术后时间点和ROI总体上，PRP治疗的膝关节软骨细胞中TGF-β1表达更高（36%±29%对15%±18%）（P = 1.8×10⁻⁶）。
与接受AOT和生理盐水治疗的兔子相比，接受AOT和PRP治疗的兔子关节软骨中TGF-β1表达增加。我们的研究结果表明，辅助使用PRP可能会增加TGF-β1表达，这可能在PRP的体内软骨生成作用中发挥作用。
