Smyth Niall A, Haleem Amgad M, Ross Keir A, Hannon Charles P, Murawski Christopher D, Do Huong T, Kennedy John G
Hospital for Special Surgery, New York, NY, USA.
Hospital for Special Surgery, New York, NY, USA; University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Cartilage. 2016 Jan;7(1):104-11. doi: 10.1177/1947603515599190.
The purpose of this study was to assess the effect(s) of platelet-rich plasma (PRP) on osteochondral donor site healing in a rabbit model.
Osteochondral donor sites 3 mm in diameter and 5 mm in depth were created bilaterally on the femoral condyles of 12 New Zealand White rabbits. Knees were randomized such that one knee in each rabbit received an intra-articular injection of PRP and the other received saline (placebo). Rabbits were euthanized at 3, 6, and 12 weeks following surgery. Repair tissue was evaluated using the International Cartilage Repair Society (ICRS) macroscopic and histological scores.
No complications occurred as a result of the interventions. There was no significant difference in macroscopic scores between the 2 groups (5.5 ± 3.8 vs. 3.8 ± 3.5; P = 0.13). Subjective macroscopic assessment determined greater tissue infill with fewer fissures and a more cartilage-like appearance in PRP-treated knees. Overall ICRS histological scores were better in the PRP group compared with the placebo (9.8 ± 2.0 vs. 7.8 ± 1.8; P = 0.04). Histological scores were also higher in the PRP group compared with the placebo group at each time point. Greater glycosaminoglycan and type II collagen content were noted in the repair tissue of the PRP group compared with the placebo group.
The results of this study indicate that PRP used as an intra-articular injection may improve osteochondral healing in a rabbit model.
本研究旨在评估富血小板血浆（PRP）对兔模型中骨软骨供体部位愈合的影响。
在12只新西兰白兔的股骨髁上双侧制备直径3mm、深度5mm的骨软骨供体部位。将膝关节随机分组，使得每只兔子的一个膝关节接受关节内注射PRP，另一个接受生理盐水（安慰剂）。术后3、6和12周对兔子实施安乐死。使用国际软骨修复协会（ICRS）的宏观和组织学评分评估修复组织。
干预未导致并发症。两组间宏观评分无显著差异（5.5±3.8对3.8±3.5；P=0.13）。主观宏观评估显示，PRP治疗的膝关节组织填充更多，裂缝更少，外观更类似软骨。与安慰剂组相比，PRP组的总体ICRS组织学评分更好（9.8±2.0对7.8±1.8；P=0.04）。在每个时间点，PRP组的组织学评分也高于安慰剂组。与安慰剂组相比，PRP组修复组织中的糖胺聚糖和II型胶原蛋白含量更高。
本研究结果表明，关节内注射PRP可能改善兔模型中的骨软骨愈合。
