Yao Pingbo, Zhao Hong, Mo Wenjuan, He Pingping
1 ICU of the Affiliated Nanhua Hospital of University of South China , Hengyang, China .
2 Nursing College, University of South China , Hengyang, China .
DNA Cell Biol. 2016 Mar;35(3):118-23. doi: 10.1089/dna.2015.3041. Epub 2015 Dec 30.
Laminar shear stress is considered to improve endothelial cell (EC) function. However, the underlying mechanism is unclear. Autophagy has been found to protect cell survival under stress. In this study, the effect of laminar shear stress on EC autophagy and its potential mechanism were explored. The autophagic markers, Beclin 1 and LC3 II, in human umbilical vascular endothelial cells increased after laminar shear stress treatment. Meanwhile, the autophagic substrate, p62, decreased. The protein level of Rab4 increased under laminar shear stress. When pretreated with Rab4 siRNA, the increased levels of Beclin 1 and LC3 II were attenuated and p62 levels significantly increased. In addition, the MCP level and the adhesion of monocytes were also obviously increased by Rab4 siRNA. Laminar shear stress upregulated Rab4 expression, which contributed to improved EC autophagy and function.
层流切应力被认为可改善内皮细胞（EC）功能。然而，其潜在机制尚不清楚。自噬已被发现可在应激状态下保护细胞存活。在本研究中，探讨了层流切应力对EC自噬的影响及其潜在机制。人脐静脉血管内皮细胞经层流切应力处理后，自噬标志物Beclin 1和LC3 II增加。同时，自噬底物p62减少。层流切应力作用下Rab4的蛋白水平升高。用Rab4 siRNA预处理后，Beclin 1和LC3 II的升高水平减弱，p62水平显著升高。此外，Rab4 siRNA还使单核细胞趋化蛋白水平及单核细胞黏附明显增加。层流切应力上调Rab4表达，这有助于改善EC自噬和功能。
