Jesionek Wioleta, Fornal Emilia, Majer-Dziedzic Barbara, Móricz Ágnes M, Nowicky Wassil, Choma Irena M
Maria Curie-Skłodowska University, Department of Chromatographic Methods, Lublin, Poland.
Laboratory of Separation and Spectroscopic Method Applications, Center for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Lublin, Poland.
J Chromatogr A. 2016 Jan 15;1429:340-7. doi: 10.1016/j.chroma.2015.12.015. Epub 2015 Dec 8.
The greater celandine (Chelidonium majus L.) has been known for the centuries as a medicinal plant. One of the therapeutic agents based on C. majus is anticancer drug Ukrain™ known as a semi-synthetic C. majus alkaloid derivative. Although there are no doubts about antitumor properties of the drug, there is still controversy about its composition. In this study, Ukrain™ was subjected to TLC and LC-MS/MS analyses to compare it with C. majus alkaloid root extract and to determine its composition. Moreover, microbiological activity of both Ukrain™ and the alkaloid extract were tested against Bacillus subtilis strains using TLC-direct bioautography. Sanguinarine, chelidonine, α-homochelidonie and chelerythrine were found to have antibacterial properties. Besides chelidonine, sanguinarine, chelerythrine, protopine, allocryptopine, homochelidonie, berberine and coptisine reported earlier in literature, the presence of stylopine, norchelidonine, dihydrochelidonine and hydroberberine in Ukrain™ was detected, and here they have been reported for the first time.
白屈菜（Chelidonium majus L.）几个世纪以来一直作为药用植物为人所知。一种基于白屈菜的治疗剂是抗癌药物 Ukrain™，它是一种半合成的白屈菜生物碱衍生物。尽管该药物的抗肿瘤特性毋庸置疑，但其成分仍存在争议。在本研究中，对 Ukrain™ 进行了薄层色谱（TLC）和液相色谱 - 串联质谱（LC - MS/MS）分析，以将其与白屈菜生物碱根提取物进行比较并确定其成分。此外，使用薄层色谱直接生物自显影法测试了 Ukrain™ 和生物碱提取物对枯草芽孢杆菌菌株的微生物活性。发现血根碱、白屈菜碱、α - 高白屈菜酸和白屈菜红碱具有抗菌特性。除了文献中先前报道的白屈菜碱、血根碱、白屈菜红碱、原阿片碱、别隐品碱、高白屈菜酸、小檗碱和黄连碱外，还检测到 Ukrain™ 中存在斯氏紫堇碱、去甲白屈菜碱、二氢白屈菜碱和氢化小檗碱，此处为首次报道。
