Sowa Ireneusz, Zielińska Sylwia, Sawicki Jan, Bogucka-Kocka Anna, Staniak Michał, Bartusiak-Szcześniak Ewa, Podolska-Fajks Maja, Kocjan Ryszard, Wójciak-Kosior Magdalena
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Pharmaceutical Biology, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
J Anal Methods Chem. 2018 Feb 20;2018:9624327. doi: 10.1155/2018/9624327. eCollection 2018.
L. is a rich source of isoquinoline alkaloids with confirmed anti-inflammatory, choleretic, spasmolytic, antitumor, and antimicrobial activities. However, their chromatographic analysis is difficult because they may exist both in charged and uncharged forms and may result in the irregular peak shape and the decrease in chromatographic system efficacy. In the present work, the separation of main alkaloids was optimized using a new-generation XB-C18 endcapped core-shell column dedicated for analysis of alkaline compounds. The influence of organic modifier concentration, addition of salts, and pH of eluents on chromatographic parameters such as retention, resolution, chromatographic plate numbers, and peak asymmetry was investigated. The results were applied to elaborate the optimal chromatographic system for simultaneous quantification of seven alkaloids from the root, herb, and fruit of .
L.是异喹啉生物碱的丰富来源，具有已证实的抗炎、利胆、解痉、抗肿瘤和抗菌活性。然而，它们的色谱分析很困难，因为它们可能以带电和不带电两种形式存在，可能导致峰形不规则以及色谱系统效能下降。在本工作中，使用新一代专门用于分析碱性化合物的XB-C18封端核壳柱对主要生物碱的分离进行了优化。研究了有机改性剂浓度、盐的添加以及洗脱液pH对保留、分离度、色谱板数和峰不对称性等色谱参数的影响。将结果应用于精心设计用于同时定量来自[植物名称]根、草和果实中七种生物碱的最佳色谱系统。
