Qu Xiaosheng, Fan LanLan, Zhong Taozheng, Li Gang, Xia Xianghua, Long Hairong, Huang Danna, Shu Wei
Engineering Lab of Endangered Medicinal Resources in Southwest China, Medicinal Plants Garden of Guangxi Zhuang Autonomous Region, 530023, China.
School of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530200, China.
Toxicon. 2016 Feb;110:74-8. doi: 10.1016/j.toxicon.2015.12.011. Epub 2015 Dec 21.
The present work investigated the effects of the nematocysts venom (NV) from the Chrysaora helvola Brandt (C. helvola) jellyfish on the human nasopharyngeal carcinoma cell line, CNE-2. The medium lethal concentration (LC50), quantified by MTT assays, was 1.7 ± 0.53 μg/mL (n = 5). An atypical apoptosis-like cell death was confirmed by LDH release assay and Annexin V-FITC/PI staining-based flow cytometry. Interestingly, activation of caspase-4 other than caspase-3, -8, -9 and -1 was observed. Moreover, the NV stimuli caused a time-dependent loss of mitochondrial membrane potential (ΔΨm) as was an intracellular ROS burst. These results indicated that there was uncoupling of oxidative phosphorylation (UOP). An examination of the intracellular pH value by a pH-sensitive fluorescent probe, BCECF, suggested that the UOP was due to the time-dependent increase in the intracellular pH. This is the first report that jellyfish venom can induce UOP.
本研究调查了黄斑海蜇（Chrysaora helvola Brandt，简称C. helvola）水母的刺丝囊毒液（NV）对人鼻咽癌细胞系CNE-2的影响。通过MTT法测定的半数致死浓度（LC50）为1.7±0.53μg/mL（n = 5）。通过乳酸脱氢酶（LDH）释放试验和基于膜联蛋白V-FITC/碘化丙啶（PI）染色的流式细胞术证实了非典型的凋亡样细胞死亡。有趣的是，观察到除了半胱天冬酶-3、-8、-9和-1之外，半胱天冬酶-4也被激活。此外，NV刺激导致线粒体膜电位（ΔΨm）随时间依赖性丧失，同时细胞内活性氧（ROS）爆发。这些结果表明存在氧化磷酸化解偶联（UOP）。用pH敏感荧光探针BCECF检测细胞内pH值表明，UOP是由于细胞内pH值随时间依赖性增加所致。这是关于水母毒液可诱导UOP的首次报道。
