Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory of Marine Drugs and Biological Products, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Toxins (Basel). 2019 Mar 10;11(3):156. doi: 10.3390/toxins11030156.
Jellyfish envenomations result in extensive dermatological symptoms, clinically named as jellyfish dermatitis, which can seriously affect the daily activities and physical health of people. Inflammatory response accompanies the whole process of jellyfish dermatitis and the complexity of jellyfish venom components makes it difficult to treat jellyfish dermatitis symptoms effectively. Moreover, inhibiting inflammation is essential for the treatment of jellyfish stings and exploring the main components of jellyfish venom that cause inflammation is an urgent research area. In this study, the inhibitory effects of matrix metalloproteinase (MMP) inhibitors for venom-induced inflammation were explored at a cellular level. The expression of the three inflammatory factors, IL-6, TNF-α and MCP-1 in two skin cell lines, human keratinocyte cells (HaCaT) and human embryonic skin fibroblasts cells (CCC-ESF-1), at the cellular level, after treatment with the inhibitors of jellyfish () nematocyst venom (NnNV-I), were determined. The results showed that inhibitors of MMP can significantly reduce the toxic effects of jellyfish nematocyst venom (NnNV) to skin cells. The expression levels of the three inflammatory factors IL-6, MCP-1, and TNF-α in the cells were also significantly decreased, indicating that MMPs in jellyfish venom are probably vital factors leading to jellyfish dermatitis. This study is beneficial in the prevention and treatment of jellyfish stings.
水母蜇伤会导致广泛的皮肤科症状，临床上称为水母皮炎，这会严重影响人们的日常活动和身体健康。炎症反应伴随着水母皮炎的整个过程，而水母毒液成分的复杂性使得有效治疗水母皮炎症状变得困难。此外，抑制炎症对于治疗水母蜇伤至关重要，探索导致炎症的水母毒液的主要成分是一个紧迫的研究领域。在这项研究中，在细胞水平上探索了基质金属蛋白酶 (MMP) 抑制剂对毒液引起的炎症的抑制作用。在用水母 （）刺丝囊毒液 (NnNV-I) 处理后，在两种皮肤细胞系，人角质形成细胞 (HaCaT) 和人胚胎皮肤成纤维细胞系 (CCC-ESF-1) 中，测定了三种炎症因子 IL-6、TNF-α 和 MCP-1 在细胞水平上的表达。结果表明，MMP 抑制剂能显著减轻水母刺丝囊毒液 (NnNV) 对皮肤细胞的毒性作用。细胞中三种炎症因子 IL-6、MCP-1 和 TNF-α 的表达水平也明显降低，表明 MMPs 在水母毒液中可能是导致水母皮炎的重要因素。这项研究有助于水母蜇伤的预防和治疗。
