Zhang Haiyang, Qu Yanjun, Duan Jingjing, Deng Ting, Liu Rui, Zhang Le, Bai Ming, Li Jialu, Zhou Likun, Ning Tao, Li Hongli, Ge Shaohua, Li Hua, Ying Guoguang, Huang Dingzhi, Ba Yi
Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China.
Department of Gastroenterology, Tianjin First Center Hospital, Tianjin 300192, P.R. China.
Oncol Rep. 2016 Feb;35(2):1135-46. doi: 10.3892/or.2015.4451. Epub 2015 Nov 26.
Gastric cancer is one of the most common malignant tumors worldwide; however, the efficacy of clinical treatment is limited. MicroRNAs (miRNAs) are a class of small non-coding RNAs that have been reported to play a key role in the development of cancer. They also provide novel candidates for targeted therapy. To date, in-depth studies on the molecular mechanisms of gastric cancer involving miRNAs are still absent. We previously reported that 5 miRNAs were identified as being significantly increased in gastric cancer, and the role of these miRNAs was investigated in the present study. By using bioinformatics tools, we found that more than 4,000 unique genes are potential downstream targets of gastric cancer miRNAs, and these targets belong to the protein class of nucleic acid binding, transcription factor, enzyme modulator, transferase and receptor. Pathway mapping showed that the targets of gastric cancer miRNAs are involved in the MAPK signaling pathway, pathways in cancer, the PI3K-Akt signaling pathway, the HTLV-1 signaling pathway and Ras signaling pathway, thus regulating cell growth, differentiation, apoptosis and metastasis. Analysis of the pathways related to miRNAs may provides potential drug targets for future therapy of gastric cancer.
胃癌是全球最常见的恶性肿瘤之一；然而，临床治疗的疗效有限。微小RNA（miRNA）是一类小的非编码RNA，据报道在癌症发展中起关键作用。它们也为靶向治疗提供了新的候选物。迄今为止，仍缺乏对涉及miRNA的胃癌分子机制的深入研究。我们之前报道过在胃癌中鉴定出5种显著上调的miRNA，本研究对这些miRNA的作用进行了探究。通过使用生物信息学工具，我们发现超过4000个独特基因是胃癌miRNA的潜在下游靶点，这些靶点属于核酸结合、转录因子、酶调节剂、转移酶和受体的蛋白质类别。通路映射显示，胃癌miRNA的靶点参与丝裂原活化蛋白激酶（MAPK）信号通路、癌症相关通路、磷脂酰肌醇-3激酶（PI3K）-蛋白激酶B（Akt）信号通路、人类嗜T淋巴细胞病毒1型（HTLV-1）信号通路和Ras信号通路，从而调节细胞生长、分化、凋亡和转移。对与miRNA相关通路的分析可能为未来胃癌治疗提供潜在的药物靶点。
