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骨样羟基磷灰石/聚氨基酸复合材料的生物学特性及其对长骨缺损修复影响的研究

Study of bone-like hydroxyapatite/polyamino acid composite materials for their biological properties and effects on the reconstruction of long bone defects.

作者信息

Yan Ling, Jiang Dian-ming

机构信息

Department of Orthopedics Surgery, The First People's Hospital of Zunyi City, Zunyi, People's Republic of China.

Department of Orthopedic Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, People's Republic of China.

出版信息

Drug Des Devel Ther. 2015 Dec 17;9:6497-508. doi: 10.2147/DDDT.S96207. eCollection 2015.

DOI:10.2147/DDDT.S96207
PMID:26719675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4687625/
Abstract

PURPOSE

The purpose of this study was to investigate the effect of bone-like hydroxyapatite/polyamino acid (BHA/PAA) in the osteogenesis and reconstruction of long segmental bone defects.

METHODS

In vitro, MG63 cells were cultured with BHA/PAA. The osteoinductive activity of the BHA/PAA material was evaluated using inverted microscopy, scanning electron microscopy, MTT proliferation assay, and the determination of alkaline phosphatase activity and Ca(2+) content. In vivo, the radial bone defect was made in 20 New Zealand White rabbits, and then these animal were randomly divided into two groups (n=10), the experimental group (with BHA/PAA) and the control group (without BHA/PAA). Postoperatively, the osteogenesis effect of BHA/PAA was evaluated through X-ray, hematoxylin-eosin staining, observation of the gross bone specimen, immunohistochemistry, and fluorescent confocal scanning microscopy.

RESULTS

In vitro, BHA/PAA promoted the adhesion, growth, and calcium nodule formation of MG63 cells, and it had good osteogenesis activity. In vivo, with BHA/PAA material degradation and absorption, the new bone gradually formed, and the bone defect gradually recovered in the experimental group. In the control group, a limited bone formation was found at the bone broken ends, and the bone defect was obviously visible.

CONCLUSION

In vitro and in vivo, we confirmed that BHA/PAA was effective in inducing osteogenesis and reconstructing a long segmental bone defect.

摘要

目的

本研究旨在探讨骨样羟基磷灰石/聚氨基酸（BHA/PAA）在长节段骨缺损成骨及修复中的作用。

方法

体外实验中，将MG63细胞与BHA/PAA共同培养。采用倒置显微镜、扫描电子显微镜、MTT增殖试验以及碱性磷酸酶活性和Ca(2+)含量测定等方法评估BHA/PAA材料的骨诱导活性。体内实验中，对20只新西兰白兔制造桡骨缺损，然后将这些动物随机分为两组（n = 10），即实验组（植入BHA/PAA）和对照组（未植入BHA/PAA）。术后，通过X射线、苏木精-伊红染色、大体骨标本观察、免疫组织化学以及荧光共聚焦扫描显微镜评估BHA/PAA的成骨效果。

结果

体外实验中，BHA/PAA促进了MG63细胞的黏附、生长和钙结节形成，具有良好的成骨活性。体内实验中，随着BHA/PAA材料的降解吸收，实验组新骨逐渐形成，骨缺损逐渐恢复。对照组在骨折断端发现有限的骨形成，骨缺损明显可见。

结论

体内外实验均证实BHA/PAA在诱导成骨和修复长节段骨缺损方面有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/bad391fda94b/dddt-9-6497Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/99b62f8fdb3b/dddt-9-6497Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/e1b6e7037a1c/dddt-9-6497Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/2d244c22ddd5/dddt-9-6497Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/00163f9517d8/dddt-9-6497Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/9031e8b9f7d6/dddt-9-6497Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/5da21e2dcc74/dddt-9-6497Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/74748dc29884/dddt-9-6497Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/d070df3f312e/dddt-9-6497Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/dd6a9bca89dd/dddt-9-6497Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/bad391fda94b/dddt-9-6497Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/99b62f8fdb3b/dddt-9-6497Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/e1b6e7037a1c/dddt-9-6497Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/2d244c22ddd5/dddt-9-6497Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/00163f9517d8/dddt-9-6497Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/9031e8b9f7d6/dddt-9-6497Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/5da21e2dcc74/dddt-9-6497Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/74748dc29884/dddt-9-6497Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/d070df3f312e/dddt-9-6497Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/dd6a9bca89dd/dddt-9-6497Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f60/4687625/bad391fda94b/dddt-9-6497Fig10.jpg

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本文引用的文献

1
Combinatorial Design of Hydrolytically Degradable, Bone-like Biocomposites Based on PHEMA and Hydroxyapatite.基于聚甲基丙烯酸羟乙酯（PHEMA）和羟基磷灰石的可水解降解骨样生物复合材料的组合设计
Polymer (Guildf). 2013 Jan 24;54(2):909-919. doi: 10.1016/j.polymer.2012.12.017. Epub 2012 Dec 13.
2
Fascin and CK4 as biomarkers for esophageal squamous cell carcinoma.Fascin 和 CK4 作为食管鳞癌的生物标志物。
Anticancer Res. 2011 Mar;31(3):945-52.
3
Composite films of gelatin and hydroxyapatite/bioactive glass for tissue-engineering applications.
用于长骨节段性缺损重建的生物材料的最新进展：叙述性综述。
J Orthop Translat. 2019 Oct 8;22:26-33. doi: 10.1016/j.jot.2019.09.005. eCollection 2020 May.
4
Interrelationships among hydrogen permeation, physiochemical properties and early adsorption abilities of titanium.氢渗透、物理化学性质与钛的早期吸附能力之间的关系。
J Mater Sci Mater Med. 2017 Nov 17;28(12):194. doi: 10.1007/s10856-017-6002-8.
5
Three-dimensional system enabling the maintenance and directed differentiation of pluripotent stem cells under defined conditions.三维系统可在特定条件下维持和定向分化多能干细胞。
Sci Adv. 2017 May 12;3(5):e1602875. doi: 10.1126/sciadv.1602875. eCollection 2017 May.
明胶/羟基磷灰石/生物活性玻璃复合膜在组织工程中的应用。
J Biomater Sci Polym Ed. 2010;21(8-9):1207-26. doi: 10.1163/092050609X12481751806213.
4
The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites.羟基磷灰石纳米颗粒的形状和尺寸对羟基磷灰石-PCL 复合材料涂层双相磷酸钙支架性能的影响。
Biomaterials. 2010 Jul;31(21):5498-509. doi: 10.1016/j.biomaterials.2010.03.058. Epub 2010 Apr 15.
5
Restoration of peri-implant defects in immediate implant installations by Choukroun platelet-rich fibrin and silk fibroin powder combination graft.采用富血小板纤维蛋白与丝素蛋白粉末联合移植修复即刻种植术中种植体周围骨缺损
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jun;109(6):831-6. doi: 10.1016/j.tripleo.2009.10.038. Epub 2010 Feb 16.
6
Alginate/Hydroxyapatite biocomposite for bone ingrowth: a trabecular structure with high and isotropic connectivity.用于骨向内生长的藻酸盐/羟基磷灰石生物复合材料：具有高且各向同性连通性的小梁结构。
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7
Poly (amino acid) micelle nanocarriers in preclinical and clinical studies.临床前和临床研究中的聚（氨基酸）胶束纳米载体
Adv Drug Deliv Rev. 2008 May 22;60(8):899-914. doi: 10.1016/j.addr.2007.11.010. Epub 2008 Feb 9.
8
Influence of calcium phosphate crystal assemblies on the proliferation and osteogenic gene expression of rat bone marrow stromal cells.磷酸钙晶体聚集体对大鼠骨髓基质细胞增殖和成骨基因表达的影响。
Biomaterials. 2007 Mar;28(7):1393-403. doi: 10.1016/j.biomaterials.2006.11.019. Epub 2006 Dec 12.
9
Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics.支架内部结构对大孔磷酸钙生物陶瓷体内骨形成的作用
Biomaterials. 2006 Jun;27(17):3230-7. doi: 10.1016/j.biomaterials.2006.01.031. Epub 2006 Feb 20.
10
A PLGA membrane controlling cell behaviour for promoting tissue regeneration.一种用于控制细胞行为以促进组织再生的聚乳酸-羟基乙酸共聚物（PLGA）膜。
Biomaterials. 2005 Dec;26(35):7447-56. doi: 10.1016/j.biomaterials.2005.05.055.