Parvandar-Asadollahi Kaveh, Mosavari Nader, Mayahi Mansoor
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Postal code: 61355145.
Razi Vaccine and Serum Research Institute, Karaj, Iran. Postal code: 31975/148.
Iran J Microbiol. 2015 Oct;7(5):260-4.
Avian tuberculosis is one of the most important infections affecting most species of birds. Mycobacterium avium can not only infect all species of birds, but also infect some domesticated mammals. The most crucial aspect of control and eradication scheme is identification of infection sources and transmission routs. Molecular techniques such as restriction fragment length polymorphism and pulse field gel electrophoresis have been shown to be much more discriminatory and suitable for use in the epidemiological study.
Eighty suspected pigeons to avian tuberculosis based on their clinical signs, were subjected to the study. Forty Mycobacterium avium subsp. avium isolates out of a total of 51 identified isolates were subjected to the test.
IS901-RFLP using Pvu II was successfully conducted and produced 7 patterns. The majority of isolates (60%) were RFLP type PI.1. This type was the most similar type to standard strain. However, all the patterns obtained in this study were different from the standard strain.
The result of this study indicate that these isolates probably are limited to Khuzestan region. We recommend DNA fingerprinting differentiation of non tuberculous Mycobacteria particularly Mycobacterium avium complex isolated from infected birds and human to possibly find source of infections.
禽结核病是影响大多数鸟类物种的最重要感染之一。鸟分枝杆菌不仅能感染所有鸟类物种，还能感染一些家养哺乳动物。控制和根除计划的最关键方面是识别感染源和传播途径。诸如限制性片段长度多态性和脉冲场凝胶电泳等分子技术已被证明具有更高的鉴别力，适用于流行病学研究。
对80只根据临床症状疑似患有禽结核病的鸽子进行了研究。在总共鉴定出的51株分离株中，对40株鸟分枝杆菌亚种鸟分枝杆菌分离株进行了检测。
使用Pvu II进行的IS901-RFLP成功完成，并产生了7种模式。大多数分离株（60%）为RFLP类型PI.1。该类型与标准菌株最为相似。然而，本研究中获得的所有模式均与标准菌株不同。
本研究结果表明这些分离株可能局限于胡齐斯坦地区。我们建议对非结核分枝杆菌，特别是从受感染鸟类和人类中分离出的鸟分枝杆菌复合群进行DNA指纹鉴别，以可能找到感染源。
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