使用限制性片段长度多态性作为鸟分枝杆菌菌株分型的遗传标记。
Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.
作者信息
Roiz M P, Palenque E, Guerrero C, Garcia M J
机构信息
Departamento de Microbiologia, Hospital Universitario 12 de Octubre, Madrid, Spain.
出版信息
J Clin Microbiol. 1995 May;33(5):1389-91. doi: 10.1128/jcm.33.5.1389-1391.1995.
Abstract
Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.
摘要
限制性片段长度多态性（RFLP）被用于研究75株鉴定为鸟分枝杆菌的临床分离株。使用两个重复插入序列IS1311和IS900作为DNA探针。尽管不到25%的分离株显示出与IS900的RFLP模式，但所有菌株都给出了与IS1311的条带模式。从艾滋病患者中分离出的鸟分枝杆菌菌株在两种探针上均表现出明显的多态性。
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