Guerrero C, Bernasconi C, Burki D, Bodmer T, Telenti A
Institute for Medical Microbiology, University of Berne, Switzerland.
J Clin Microbiol. 1995 Feb;33(2):304-7. doi: 10.1128/jcm.33.2.304-307.1995.
The insertion sequence IS1245 is a novel mycobacterial repetitive element identified in Mycobacterium avium. It encodes a transposase which exhibits a 64% amino acid similarity with IS1081, an insertion element present in the M. tuberculosis complex. The host range of IS1245 appears limited to M. avium as this element was not identified in M. intracellulare or in any other of 18 mycobacteria species tested. When IS1245 was used for restriction fragment length polymorphism (RFLP) analysis, human isolates characteristically presented a high number of copies (median, 16; range, 3 to 27) and a diversity of RFLP patterns comparable to that found by pulsed-field gel electrophoresis. Isolates from nonhuman sources differed both in number of copies and in RFLP pattern diversity: while swine isolates shared the characteristics of human strains, those from several avian sources exhibited a very low copy number of IS1245 and appeared clonal on the basis of RFLP.
插入序列IS1245是在鸟分枝杆菌中鉴定出的一种新型分枝杆菌重复元件。它编码一种转座酶，该转座酶与结核分枝杆菌复合群中存在的插入元件IS1081具有64%的氨基酸相似性。IS1245的宿主范围似乎仅限于鸟分枝杆菌，因为在胞内分枝杆菌或所测试的其他18种分枝杆菌中均未鉴定到该元件。当将IS1245用于限制性片段长度多态性（RFLP）分析时，人类分离株通常呈现出大量的拷贝数（中位数为16；范围为3至27），并且RFLP模式的多样性与脉冲场凝胶电泳所发现的相当。来自非人类来源的分离株在拷贝数和RFLP模式多样性方面均有所不同：猪分离株具有人类菌株的特征，而来自几种禽类来源的分离株IS1245拷贝数非常低，并且基于RFLP显示为克隆性。
