Ghasemi Behzad, Sanjarani Ghasem, Sanjarani Zahra, Majidiani Hamidreza
Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Iran J Microbiol. 2015 Oct;7(5):281-6.
Bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. Thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella typhimurium, Proteus mirabilis and Shigella dysenteriae.
The thiazole and imidazole derivatives were dissolved in DMSO. The disk diffusion method was utilized to measure the growth inhibition zone diameter values, and the broth micro-dilution method was applied to determine the minimum inhibitory concentration (MIC) values.
The synthesized imidazole derivatives lacked any inhibitory effect against the tested bacteria. On the other hand, although the synthesized thiazole derivative showed no inhibitory effect against Bacillus cereus, Salmonella typhimurium, and Escherichia coli, it inhibited the growth of Proteus mirabilis, Shigella dysenteriae, and Listeria monocytogenes with the MIC values of 1000, 125, and 1000 μg/ml, respectively, and the growth inhibition zone diameter values of 9.3 ± 0.1, 15.6 ± 0.2, and 8.1 ± 0.0 mm, respectively.
The anti-bacterial effect of the synthesized thiazole derivative on Shigella dysenteriae, Proteus mirabilis and Listeria monocytogenes was proven. However, its inhibition effect against Shigella dysenteriae was more than that against the others. Many in-vitro and in-vivo experiments are required to evaluate the effects of this compound on the bacteria and the human body.
细菌对抗生素的耐药性促使研究人员评估新型抗菌化合物，如一些噻唑和咪唑衍生物。因此，在本研究中，我们研究了一种新型噻唑和两种新型咪唑衍生物对蜡样芽孢杆菌、单核细胞增生李斯特菌、大肠杆菌、鼠伤寒沙门氏菌、奇异变形杆菌和痢疾志贺氏菌的抗菌作用。
将噻唑和咪唑衍生物溶解于二甲基亚砜（DMSO）中。采用纸片扩散法测量抑菌圈直径值，并用肉汤微量稀释法测定最低抑菌浓度（MIC）值。
合成的咪唑衍生物对受试细菌均无抑制作用。另一方面，尽管合成的噻唑衍生物对蜡样芽孢杆菌、鼠伤寒沙门氏菌和大肠杆菌无抑制作用，但它能抑制奇异变形杆菌、痢疾志贺氏菌和单核细胞增生李斯特菌的生长，其MIC值分别为1000、125和1000μg/ml，抑菌圈直径值分别为9.3±0.1、15.6±0.2和8.1±0.0mm。
已证实合成的噻唑衍生物对痢疾志贺氏菌、奇异变形杆菌和单核细胞增生李斯特菌有抗菌作用。然而，其对痢疾志贺氏菌的抑制作用比对其他细菌的抑制作用更强。需要进行许多体外和体内实验来评估该化合物对细菌和人体的影响。
