新型口腔喷雾肽对. 的抗菌作用
Antimicrobial Effect of a Peptide Containing Novel Oral Spray on .
机构信息
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservation Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Biomed Res Int. 2020 Mar 10;2020:6853652. doi: 10.1155/2020/6853652. eCollection 2020.
OBJECTIVE
To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on and further explore the related mechanisms at phenotypic and transcriptional levels.
METHODS
The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards was determined by the broth dilution method. was determined by the broth dilution method.
RESULTS
The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria was determined by the broth dilution method. was determined by the broth dilution method. was determined by the broth dilution method. was determined by the broth dilution method. , , , and were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results.
CONCLUSION
The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti- effect and the inhibitory property may be due to suppression of the virulence factors of including adhesive, acidogenicity, EPS, and biofilm formation. effect and the inhibitory property may be due to suppression of the virulence factors of including adhesive, acidogenicity, EPS, and biofilm formation. was determined by the broth dilution method.
目的
研究一种新型含抗菌肽口腔喷雾 GERM CLEAN 对 的抗菌作用，并从表型和转录水平进一步探讨其相关机制。
方法
采用圆盘扩散法初步评价 GERM CLEAN 的抗菌效果。采用肉汤稀释法测定 GERM CLEAN 对 的最小抑菌浓度（MIC）。
结果
GERM CLEAN 形成的抑菌圈直径（10.18±1.744mm）初步表明其对主要致龋菌 的抑制作用。采用肉汤稀释法测定 GERM CLEAN 对 的 MIC 值。GERM CLEAN 能显著抑制 、 、 和 的生长，这与我们的表型结果一致。
结论
新型含抗菌肽口腔喷雾 GERM CLEAN 具有抗 作用，其抑制特性可能是通过抑制 的毒力因子，包括黏附性、产酸、EPS 和生物膜形成。
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