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克隆编码1-氨基环丙烷-1-羧酸合酶的信使核糖核酸，该酶是植物乙烯生物合成的关键酶。

Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants.

作者信息

Sato T, Theologis A

机构信息

Agricultural Research Service, U.S. Department of Agriculture, Albany, CA 94710.

出版信息

Proc Natl Acad Sci U S A. 1989 Sep;86(17):6621-5. doi: 10.1073/pnas.86.17.6621.

DOI:10.1073/pnas.86.17.6621
PMID:2671999
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC297896/
Abstract

Ethylene is the plant hormone that controls several features of plant growth and development. The rate-limiting step in its synthesis is the formation of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) from S-adenosylmethionine (AdoMet), catalyzed by ACC synthase. We have isolated a complementary DNA sequence encoding ACC synthase from zucchini (Cucurbita) fruits. The biological activity of the clone was confirmed by the ability of the cloned sequence to direct ACC synthase activity in Escherichia coli and yeast. In vivo studies using the ACC cDNA as probe showed that the ACC synthase gene is induced by a diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin.

摘要

乙烯是一种控制植物生长和发育多个特征的植物激素。其合成中的限速步骤是由ACC合酶催化，从S-腺苷甲硫氨酸（AdoMet）形成乙烯前体1-氨基环丙烷-1-羧酸（ACC）。我们从西葫芦（南瓜属）果实中分离出了一个编码ACC合酶的互补DNA序列。克隆序列在大肠杆菌和酵母中指导ACC合酶活性的能力证实了该克隆的生物活性。使用ACC cDNA作为探针的体内研究表明，ACC合酶基因受到多种诱导剂的诱导，包括创伤、锂离子和植物激素生长素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/ddc2caf5ce23/pnas00284-0193-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/e60ef676e5ce/pnas00284-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/0cf512d63d91/pnas00284-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/5abe8fa33c34/pnas00284-0191-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/3b8d58bbe878/pnas00284-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/a3f951a7a235/pnas00284-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/49bd0132b6a1/pnas00284-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/ddc2caf5ce23/pnas00284-0193-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/e60ef676e5ce/pnas00284-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/0cf512d63d91/pnas00284-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/5abe8fa33c34/pnas00284-0191-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/3b8d58bbe878/pnas00284-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/a3f951a7a235/pnas00284-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/49bd0132b6a1/pnas00284-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4444/297896/ddc2caf5ce23/pnas00284-0193-c.jpg

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