不同生物炭对鸡粪堆肥过程中抗生素抗性基因和细菌群落的影响。
Effect of different biochars on antibiotic resistance genes and bacterial community during chicken manure composting.
机构信息
Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
出版信息
Bioresour Technol. 2016 Mar;203:11-7. doi: 10.1016/j.biortech.2015.12.030. Epub 2015 Dec 19.
Rice straw biochar (RSB) and mushroom biochar (MB) were added to lab-scale chicken manure composting to evaluate their effects on the behaviors of antibiotic resistance genes (ARGs) and on total and bio-available heavy metals (Cu, Zn and As). The associated bacterial community was characterized by 16SrRNA high-throughput sequencing. The abundance of pathogenic bacteria was also calculated. At the end of the control composting experiment, the average removal rate of ARGs was 0.86 log units and the removal rate of pathogenic bacteria was 57.1%. MB addition resulted in a higher removal rate than that in the control composting experiment. However, RSB addition yielded opposite results, which may be due to the higher abundance of Erysipelotrichaceae, Lactobacillaceae, Family_XI_Incertae_Sedis (belonging to Firmicutes carrying and disseminating ARGs) and pathogenic bacteria carrying ARGs. Furthermore, the correlations between bio-available heavy metals and ARGs were more obvious than those between total heavy metals and ARGs.
稻草生物炭（RSB）和蘑菇生物炭（MB）被添加到实验室规模的鸡粪堆肥中，以评估它们对抗生素抗性基因（ARGs）和总生物可利用重金属（Cu、Zn 和 As）行为的影响。通过 16SrRNA 高通量测序对相关细菌群落进行了表征。还计算了致病菌的丰度。在对照堆肥实验结束时，ARGs 的平均去除率为 0.86 个对数单位，致病菌的去除率为 57.1%。MB 的添加比对照堆肥实验的去除率更高。然而，RSB 的添加却产生了相反的结果，这可能是由于 Erysipelotrichaceae、Lactobacillaceae、Family_XI_Incertae_Sedis（属于携带和传播 ARGs 的 Firmicutes）和携带 ARGs 的致病菌的丰度较高所致。此外，生物可利用重金属与 ARGs 之间的相关性比总重金属与 ARGs 之间的相关性更明显。
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