EGFL7-RNAi对胶质瘤内皮血管生成的体外抑制作用
In-vitro inhibitory effect of EGFL7-RNAi on endothelial angiogenesis in glioma.
作者信息
Li Qiang, Wang Ai-Yue, Xu Qiong-Guan, Liu Da-Yuan, Xu Peng-Xiang, Yu Dai
机构信息
Department of Neurosurgery, Hainan Nongken Hospital Haikou, Hainan, China.
Department of Neurology, Haikou Municipal People's Hospital Haikou, Hainan, China.
出版信息
Int J Clin Exp Pathol. 2015 Oct 1;8(10):12234-42. eCollection 2015.
OBJECTIVE
To investigate the role and mechanism of epidermal growth factor like domain 7 (EGFL7) in glioma angiogenesis by cell co-culture and RNA interference.
METHODS
NSCs-HUVECs co-culture system was established using Transwell culturing techniques. The interactions between glioma and endothelial cells were simulated in-vitro. Cellular expression of EGFL7 in NSCs and HUVEC was targeted and suppressed by lentiviral vector carrying siRNA. The effect of EGFL7 on angiogenesis in glioma in-vitro micro-environment was detected by endothelial cell proliferation, adhesion and tube formation assay.
RESULTS
Following EGFL7 gene silencing, expression of EGFL7 in HUVECs was reduced and cell adhesion capability was inhibited significantly. Endothelial cells failed to form a lumen-like structure after EGFL7 gene silencing, shown by the tube formation assay.
CONCLUSION
By regulating endothelial cell adhesion, EGFL7 plays a key role in the regulation of glioma angiogenesis.
目的
通过细胞共培养和RNA干扰研究表皮生长因子样结构域7（EGFL7）在胶质瘤血管生成中的作用及机制。
方法
采用Transwell培养技术建立神经干细胞-人脐静脉内皮细胞（NSCs-HUVECs）共培养体系。体外模拟胶质瘤与内皮细胞之间的相互作用。携带小干扰RNA（siRNA）的慢病毒载体靶向抑制神经干细胞和人脐静脉内皮细胞中EGFL7的细胞表达。通过内皮细胞增殖、黏附及管腔形成实验检测EGFL7对胶质瘤体外微环境中血管生成的影响。
结果
EGFL7基因沉默后，人脐静脉内皮细胞中EGFL7的表达降低，细胞黏附能力明显受到抑制。管腔形成实验显示，EGFL7基因沉默后内皮细胞未能形成管腔样结构。
结论
EGFL7通过调节内皮细胞黏附在胶质瘤血管生成调控中起关键作用。
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