Hou Xu, Qiao Haiquan
Department of General Surgery, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
Int J Clin Exp Pathol. 2015 Oct 1;8(10):13108-13. eCollection 2015.
Gastric cancer pathogenesis is a multi-factor, multi-step, complicated process that related to gene abnormal expression. This study intended to explore the miR-340 effect on human gastric cancer cell line SGC-7901 and BGG823 proliferation and apoptosis, as to provide theoretical basis and experimental evidence for potential clinical application. Array was used to screen gastric cancer related abnormal genes. Q-PCR was applied to detect the screened genes expression in tissue and gastric cancer cells. MTT and colony formation assay were performed to evaluate miR-340 impact on gastric cancer proliferation. Flow cytometry was used to determine cell cycle and cell apoptosis. Q-PCR showed that miR-340 overexpressed in gastric cancer tissue significantly compared with normal control (P < 0.01). MiR-340 overexpression can promote SGC-7901 and BGC823 cells proliferation with 50% proliferation rate. Soft agar colony formation assay also showed that miR-340 overexpression can facilitate gastric cancer cell proliferation. Cell cycle analysis revealed that miR-340 overexpression can reduce cell apoptosis. Annexin V/PI staining demonstrated that miR-340 transfection can decrease cell apoptotic rate (4.58%, 1.98%, 2.11%). MiR-340 can promote tumor cell growth and reduce cell apoptosis effectively.
胃癌发病机制是一个与基因异常表达相关的多因素、多步骤的复杂过程。本研究旨在探讨miR-340对人胃癌细胞系SGC-7901和BGC823增殖及凋亡的影响，为其潜在的临床应用提供理论依据和实验证据。采用基因芯片筛选与胃癌相关的异常基因。应用Q-PCR检测筛选出的基因在组织及胃癌细胞中的表达。采用MTT法和集落形成实验评估miR-340对胃癌增殖的影响。采用流式细胞术检测细胞周期及细胞凋亡情况。Q-PCR结果显示，与正常对照相比，miR-340在胃癌组织中显著高表达（P < 0.01）。miR-340过表达可使SGC-7901和BGC823细胞增殖率提高50%，促进胃癌细胞增殖。软琼脂集落形成实验也表明miR-340过表达可促进胃癌细胞增殖。细胞周期分析显示，miR-340过表达可减少细胞凋亡。Annexin V/PI染色表明，miR-340转染可降低细胞凋亡率（4.58%、1.98%、2.11%）。miR-340可有效促进肿瘤细胞生长并减少细胞凋亡。
