Huang Mingwei, Wu Liucheng, Luo Shanshan, Qin Haiquan, Yang Yang, Chen Jiansi, Li Zhao, Qin Yuzhou
Department of Gastrointestinal Surgery, Affiliated Tumor Hospital of Guangxi Medical University, No.71 Hedi Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
Biotechnol Lett. 2017 Jan;39(1):33-38. doi: 10.1007/s10529-016-2213-1. Epub 2016 Sep 15.
To explore the functional effects of miR-1284 on gastric cancer cells.
Overexpression of miR-1284 significantly reduced SGC-7901 cell proliferation, but improved apoptosis. However, miR-1284 suppression displayed the inversed impacts. Furthermore, the protein levels of p27, Bax, procaspase-3 and active caspase-3 were up-regulated by miR-1284 overexpression, but were down-regulated by miR-1284 suppression. The level of Bcl-2 was down-regulated by miR-1284 overexpression, while it was up-regulated by miR-1284 suppression. The level of p21 was unaffected.
These results suggest that miR-1284 overexpression might be a suppressor for gastric cancer via controlling of cell proliferation and apoptosis.
探讨miR - 1284对胃癌细胞的功能影响。
miR - 1284过表达显著降低SGC - 7901细胞增殖，但促进细胞凋亡。然而，抑制miR - 1284则产生相反的影响。此外，miR - 1284过表达上调p27、Bax、procaspase - 3和活性caspase - 3的蛋白水平，而抑制miR - 1284则使其下调。miR - 1284过表达下调Bcl - 2水平，而抑制miR - 1284则使其上调。p21水平不受影响。
这些结果表明，miR - 1284过表达可能通过控制细胞增殖和凋亡而成为胃癌的一种抑制因子。
