土耳其马尔马拉地区南部缺血性中风患者的肿瘤坏死因子α、白细胞介素10和白细胞介素6基因多态性
Tumour necrosis factor alpha, ınterleukin 10 and ınterleukin 6 gene polymorphisms of ıschemic stroke patients ın south Marmara region of Turkey.
作者信息
Ozkan Adile, Sılan Fatma, Uludağ Ahmet, Degirmenci Yıldız, Ozisik Karaman Handan Isin
机构信息
Department of Neurology, Canakkale 18 Mart University Medical Faculty Canakkale, Turkey.
Department of Genetics, Canakkale 18 Mart University Medical Faculty Canakkale, Turkey.
出版信息
Int J Clin Exp Pathol. 2015 Oct 1;8(10):13500-4. eCollection 2015.
BACKGROUND
Stroke is an important cause of adult mortality and morbidity; however its pathogenesis is still unknown. Several studies have examined to determine the role of genetic polymorphism of proinflammatory cytokines in the occurence of stroke. The objective of this study was to evaluate the relationship between three polymorphisms; including tumour necrosis alpha (TNFα)-238 GA, interleukin( IL-10)-1028 GA (rs1800896), IL-6-(rs1800795) and ischemic stroke in a Turkish population.
METHODS
Forty two stroke patients and 48 healhty controls were genotyped using PCR analysis for TNFα-238 G/A, IL-10-1028 GA and IL-6-rs1800795 AG polymorphisms.
RESULTS
The frequency of the CC and CG, GG genotype of IL-6 gene (rs1800795) were statiscially significiantly higher in IS patients than controls (for C/C genotype, P=0.03, OR=4.3; 95% CI: 1.13 to 16.29 and for C/G genotype, P=0.04, OR=3.6; 95% CI: 1.03 to 12.95, for G/G genotype, P=0.02, OR=0.25; 95% CI: 0.07-0.85 respectively).
CONCLUSION
Il-6 CC genotyped was found strongly associated with ischemic stroke than other two polymorpisms TNF-α and IL-10 in our population.
背景
中风是成人死亡和发病的重要原因；然而其发病机制仍不清楚。多项研究已进行检测以确定促炎细胞因子基因多态性在中风发生中的作用。本研究的目的是评估三种多态性之间的关系；包括肿瘤坏死因子α（TNFα）-238 GA、白细胞介素（IL-10）-1028 GA（rs1800896）、IL-6-（rs1800795）与土耳其人群缺血性中风之间的关系。
方法
采用聚合酶链反应（PCR）分析法对42例中风患者和48例健康对照者进行TNFα-238 G/A、IL-10-1028 GA和IL-6-rs1800795 AG多态性基因分型。
结果
IL-6基因（rs1800795）的CC和CG、GG基因型频率在缺血性中风患者中显著高于对照组（对于C/C基因型，P = 0.03，OR = 4.3；95%可信区间：1.13至16.29；对于C/G基因型，P = 0.04，OR = 3.6；95%可信区间：1.03至12.95；对于G/G基因型，P = 0.02，OR = 0.25；95%可信区间：0.07 - 0.85）。
结论
在我们的人群中，发现IL-6 CC基因型与缺血性中风的相关性比其他两种多态性TNF-α和IL-10更强。
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