Lee Byung-Cheol, Ahn Se-Young, Doo Ho-Kyung, Yim Sung-Vin, Lee Hee-Jae, Jin Sheng-Yu, Hong Seung-Jae, Lee Sang-Ho, Kim Sung-Do, Seo Jung-Chul, Leem Kang-Hyun, Chung Joo-Ho
Department of Oriental Internal Medicine, College of Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Ku, Seoul 130-701, South Korea.
Neurosci Lett. 2004 Feb 26;357(1):33-6. doi: 10.1016/j.neulet.2003.12.041.
Enhanced release of proinflammatory cytokines may contribute to the pathogenesis of ischemic stroke. Interleukin-1 receptor antagonist (IL-1Ra) is an anti-inflammatory cytokine, and tumor necrosis factor (TNF)-alpha and IL-1beta are proinflammatory cytokine. To determine the role of cytokines in genetic susceptibility to ischemic stroke, we genotyped ischemic stroke patients (n = 152) and the healthy control subjects (n = 165) for IL-1Ra, TNF-alpha and IL-1beta polymorphism by polymerase chain reaction-restriction fragment length polymorphism methods. The analysis shown the association of IL1RN1, IL1RN2 allele (IL1RN1, OR=0.44, P = 0.0206 IL1RN2, OR=2.90, P = 0.0141) and TNF1, TNF2 allele (TNF1, OR=2.16, P = 0.0225; TNF2, OR=2.16, P = 0.0225) to ischemic stroke. However, the genetic polymorphism of IL-1beta was not associated with ischemic stroke. Our results suggest that IL-1Ra and TNF-alpha gene polymorphism is associated with the susceptibility to ischemic stroke.
促炎细胞因子的释放增加可能与缺血性中风的发病机制有关。白细胞介素-1受体拮抗剂（IL-1Ra）是一种抗炎细胞因子，而肿瘤坏死因子（TNF）-α和IL-1β是促炎细胞因子。为了确定细胞因子在缺血性中风遗传易感性中的作用，我们采用聚合酶链反应-限制性片段长度多态性方法，对152例缺血性中风患者和165例健康对照者进行了IL-1Ra、TNF-α和IL-1β基因多态性的基因分型。分析显示IL1RN1、IL1RN2等位基因（IL1RN1，OR=0.44，P = 0.0206；IL1RN2，OR=2.90，P = 0.0141）以及TNF1、TNF2等位基因（TNF1，OR=2.16，P = 0.0225；TNF2，OR=2.16，P = 0.0225）与缺血性中风有关。然而，IL-1β的基因多态性与缺血性中风无关。我们的结果表明，IL-1Ra和TNF-α基因多态性与缺血性中风的易感性有关。
