Reddy Swetha, Akgul Ali, Karsi Attila, Abdelhamed Hossam, Wills Robert W, Lawrence Mark L
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
Microb Pathog. 2016 Mar;92:19-25. doi: 10.1016/j.micpath.2015.12.012. Epub 2015 Dec 25.
Lmof2365_2117 is a Listeria monocytogenes putative cell wall surface anchor protein with a conserved domain found in collagen binding proteins. We constructed a deletion mutation in lmof2365_2117 in serotype 4b strain F2365, evaluated its virulence, and determined its ability to adhere and invade colonic epithelial cells and macrophages. In A/J mice, colonization of liver was significantly higher for F2365 than for F2365Δ2117. The ability of F2365Δ2117 to adhere to Caco-2 cells was significantly lower than F2365. The mutant also showed impaired ability to replicate in intestinal epithelial cell and murine macrophages relative to wild type F2365. Lmof2365_2117 contributed to L. monocytogenes attachment to catfish fillets. Because of its role in adherence to Caco-2 cells, we designated Lmof2365_2117 Listeria adhesion protein B (LapB). The carboxy terminus of LapB is similar to a domain in collagen binding proteins, but our results show that L. monocytogenes does not bind collagen.
Lmof2365_2117是一种单核细胞增生李斯特菌假定的细胞壁表面锚定蛋白，其具有在胶原蛋白结合蛋白中发现的保守结构域。我们在4b血清型菌株F2365的lmof2365_2117中构建了缺失突变体，评估了其毒力，并确定了其黏附并侵袭结肠上皮细胞和巨噬细胞的能力。在A/J小鼠中，F2365在肝脏中的定殖显著高于F2365Δ2117。F2365Δ2117黏附于Caco-2细胞的能力显著低于F2365。相对于野生型F2365，该突变体在肠上皮细胞和小鼠巨噬细胞中的复制能力也受损。Lmof2365_2117有助于单核细胞增生李斯特菌附着于鲶鱼片。由于其在黏附于Caco-2细胞中的作用，我们将Lmof2365_2117命名为李斯特菌黏附蛋白B（LapB）。LapB的羧基末端类似于胶原蛋白结合蛋白中的一个结构域，但我们的结果表明单核细胞增生李斯特菌不结合胶原蛋白。
