Zhang Jiawen, Zheng Peiying, Li Jing, Yang Yijing, Zeng Shaoxiao, Qiu Jianqing, Lin Shaoling
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Foods. 2022 Mar 11;11(6):808. doi: 10.3390/foods11060808.
Sono-photodynamic sterilization technology (SPDT) has become a promising non-thermal food sterilization technique because of its high penetrating power and outstanding microbicidal effects. In this study, () was effectively inactivated using curcumin as the sono-photosensitizer activated by ultrasound and blue LED light. The SPDT treatment at optimized conditions yielded a 4-log reduction in CFU. The reactive oxygen species (ROS) production in upon SPDT treatment was subsequently investigated. The results demonstrated SPDT treatment-induced excessive ROS generation led to bacterial cell deformation and membrane rupture, as revealed by the scanning electron microscope (SEM) and cytoplasmic material leakage. Moreover, agarose gel electrophoresis and SDS-PAGE further revealed that SPDT also triggered bacterial genomic DNA cleavage and protein degradation in , thus inducing bacterial apoptosis-like events, such as membrane depolarization.
声动力杀菌技术（SPDT）因其高穿透能力和出色的杀菌效果，已成为一种很有前景的非热食品杀菌技术。在本研究中，（）使用姜黄素作为超声和蓝色LED光激活的声敏剂被有效灭活。在优化条件下进行的SPDT处理使CFU减少了4个对数级。随后研究了SPDT处理后（）中活性氧（ROS）的产生。结果表明，扫描电子显微镜（SEM）和细胞质物质泄漏显示，SPDT处理诱导的过量ROS生成导致细菌细胞变形和膜破裂。此外，琼脂糖凝胶电泳和SDS-PAGE进一步表明，SPDT还引发了（）中细菌基因组DNA的裂解和蛋白质降解，从而诱导了类似细菌凋亡的事件，如膜去极化。
