Bakhtiar Ray, Guan Ziqiang
Merck Research Laboratories, Rahway, NJ 07065, USA.
Biotechnol Lett. 2006 Jul;28(14):1047-59. doi: 10.1007/s10529-006-9065-z. Epub 2006 Jun 23.
Electron capture dissociation (ECD) represents one of the most recent and significant advancements in tandem mass spectrometry (MS/MS) for the identification and characterization of polypeptides. In comparison with the conventional fragmentation techniques, such as collisionally activated dissociation (CAD), ECD provides more extensive sequence fragments, while allowing the labile modifications to remain intact during backbone fragmentation--an important attribute for characterizing post-translational modifications. Herein, we present a brief overview of the ECD technique as well as selected applications in characterization of peptides and proteins. Case studies including characterization and localization of amino acid glycosylation, methionine oxidation, acylation, and "top-down" protein mass spectrometry using ECD will be presented. A recent technique, coined as electron transfer dissociation (ETD), will be also discussed briefly.
电子捕获解离（ECD）是串联质谱（MS/MS）在多肽鉴定和表征方面的最新重大进展之一。与传统的碎片化技术（如碰撞激活解离（CAD））相比，ECD能提供更广泛的序列片段，同时在主链碎片化过程中使不稳定修饰保持完整——这是表征翻译后修饰的一个重要特性。在此，我们简要概述ECD技术以及在肽和蛋白质表征中的一些应用。将展示包括使用ECD进行氨基酸糖基化、甲硫氨酸氧化、酰化表征以及“自上而下”蛋白质质谱分析和定位的案例研究。还将简要讨论一种最新技术，即电子转移解离（ETD）。
