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草鱼（Ctenopharyngodon idella）中MicroRNA对TLR-5的负调控作用

MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella.

作者信息

Xu Xiao-Yan, Shen Yu-Bang, Fu Jian-Jun, Yu Hong-Yan, Huang Wen-Ji, Lu Li-Qun, Li Jia-Le

机构信息

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.

National Pathogen Collection Center for Aquatic Animals, College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, PR China.

出版信息

Sci Rep. 2016 Jan 4;6:18595. doi: 10.1038/srep18595.

DOI:10.1038/srep18595
PMID:26727169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4698583/
Abstract

MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in antibacterial defence in fish has not been fully determined. Here, we identified that nine miRNAs are differentially expressed in kidney between susceptible and resistant grass carp strains. Analysis of spatial and temporal miRNA expression patterns suggests that cid-miRn-115 and miR-142a-3p are potential regulators of anti-bacterial activity. Overexpressing of cid-miRn-115 and miR-142a-3p results in a visible change in Ctenopharyngodon idella kidney (CIK) cells immune effector activity. Bioinformatics analysis and overexpressing assay shows that cid-miRn-115 and miR-142a-3p directly regulate tlr5 expression. cid-miRn-115 and miR-142a-3p overexpressing leads to a significant decrease in tlr5 expression in CIK, thereby repressing its downstream genes, such as il-1β, il-8 and tnf-α. These findings provide a novel insight into the determination of anti-bacterial compounds in grass carp.

摘要

微小RNA（miRNA）是内源性小非编码RNA，在众多生物学过程中发挥关键作用。然而，miRNA在鱼类抗菌防御中的作用尚未完全确定。在此，我们鉴定出在易感和抗性草鱼品系的肾脏中，有9种miRNA差异表达。对miRNA时空表达模式的分析表明，cid-miRn-115和miR-142a-3p是抗菌活性的潜在调节因子。过表达cid-miRn-115和miR-142a-3p会导致草鱼肾脏（CIK）细胞免疫效应活性发生明显变化。生物信息学分析和过表达试验表明，cid-miRn-115和miR-142a-3p直接调节tlr5表达。过表达cid-miRn-115和miR-142a-3p会导致CIK中tlr5表达显著降低，从而抑制其下游基因，如il-1β、il-8和tnf-α。这些发现为草鱼抗菌化合物的测定提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/2dd4ae1582ad/srep18595-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/dc3883d841f5/srep18595-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/347403ff8e73/srep18595-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/e5a1342ad6e9/srep18595-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/adf92d6df838/srep18595-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/aca9050235d2/srep18595-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/2dd4ae1582ad/srep18595-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/dc3883d841f5/srep18595-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/347403ff8e73/srep18595-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/e5a1342ad6e9/srep18595-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/adf92d6df838/srep18595-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/aca9050235d2/srep18595-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96d/4698583/2dd4ae1582ad/srep18595-f6.jpg

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PLoS One. 2014 Nov 19;9(11):e112722. doi: 10.1371/journal.pone.0112722. eCollection 2014.
2
In-depth profiling and analysis of host and viral microRNAs in Japanese flounder (Paralichthys olivaceus) infected with megalocytivirus reveal involvement of microRNAs in host-virus interaction in teleost fish.对感染传染性脾肾坏死病毒的牙鲆（Paralichthys olivaceus）宿主和病毒微小RNA进行深入分析，揭示了微小RNA在硬骨鱼宿主-病毒相互作用中的作用。
BMC Genomics. 2014 Oct 8;15(1):878. doi: 10.1186/1471-2164-15-878.
3
Front Immunol. 2023 Feb 6;14:1091607. doi: 10.3389/fimmu.2023.1091607. eCollection 2023.
4
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Front Immunol. 2023 Feb 3;14:1098455. doi: 10.3389/fimmu.2023.1098455. eCollection 2023.
5
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6
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Mol Genet Genomics. 2021 Jul;296(4):765-781. doi: 10.1007/s00438-021-01786-x. Epub 2021 Apr 27.
7
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9
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