Duarte Antonia Eliene, Waczuk Emily Pansera, Roversi Katiane, da Silva Maria Arlene Pessoa, Barros Luiz Marivando, da Cunha Francisco Assis Bezerra, de Menezes Irwin Rose Alencar, da Costa José Galberto Martins, Boligon Aline Augusti, Ademiluyi Adedayo Oluwaseun, Kamdem Jean Paul, Rocha João Batista Teixeira, Burger Marilise Escobar
Centro de Ciências Biológicas e da Saúde-CCBS, Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Pimenta, Crato CEP 63.100-000, CE, Brazil.
Programa de Pós-Graduação em Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil.
Molecules. 2015 Dec 29;21(1):E2. doi: 10.3390/molecules21010002.
Raphiodon echinus (R. echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. echinus on human erythrocytes and leukocytes were investigated for the first time. R. echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 μg/mL (EtOH extract) and IC50 = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe(2+) (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30-480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.
多刺针吻鲀（Raphiodon echinus，R. echinus）在巴西民间医学中用于治疗炎症、咳嗽和传染病。然而，关于这种植物潜在的抗氧化、细胞毒性和遗传毒性尚无相关信息。在本研究中，首次对多刺针吻鲀水提取物和乙醇提取物的多酚成分、抗氧化能力以及对人红细胞和白细胞的潜在毒性作用进行了研究。多刺针吻鲀提取物中含有没食子酸、绿原酸、咖啡酸和鞣花酸、芦丁、槲皮苷和槲皮素。多刺针吻鲀水提取物和乙醇提取物在清除DPPH自由基实验中表现出抗氧化活性，乙醇提取物的IC50 = 111.9 μg/mL，水提取物的IC50 = 227.9 μg/mL。提取物抑制了铁离子（10 μM）诱导的大鼠脑和肝匀浆中硫代巴比妥酸反应性物质（TBARS）的形成。提取物（30 - 480 μg/mL）在人血细胞中未诱导遗传毒性、细胞毒性或渗透脆性。因此，本研究结果表明，多刺针吻鲀的治疗作用可能部分与其抗氧化潜力有关。然而，需要进一步的体外和体内研究来确定其在民间医学中使用的安全范围。
