Andrews Kimberly R, Good Jeffrey M, Miller Michael R, Luikart Gordon, Hohenlohe Paul A
Department of Fish and Wildlife Sciences, University of Idaho, 875 Perimeter Drive MS 1136, Moscow, Idaho 83844-1136, USA.
University of Montana, Division of Biological Sciences, 32 Campus Dr. HS104, Missoula, Montana 59812, USA.
Nat Rev Genet. 2016 Feb;17(2):81-92. doi: 10.1038/nrg.2015.28. Epub 2016 Jan 5.
High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers for any species, including non-model organisms, which is revolutionizing ecological, evolutionary and conservation genetics. Technical differences among these methods lead to important considerations for all steps of genomics studies, from the specific scientific questions that can be addressed, and the costs of library preparation and sequencing, to the types of bias and error inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq methods to aid researchers in choosing among the many different approaches and avoiding erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic marker types in the past.
基于限制性位点相关DNA测序（RADseq）的高通量技术，能够以低成本发现并对包括非模式生物在内的任何物种的数千个遗传标记进行基因分型，这正在革新生态、进化和保护遗传学。这些方法之间的技术差异，使得基因组学研究的各个步骤都需要重要考量，从能够解决的具体科学问题、文库制备和测序的成本，到所得数据中固有的偏差和误差类型。在本综述中，我们对RADseq方法进行了全面讨论，以帮助研究人员在众多不同方法中做出选择，并避免因RADseq数据得出错误的科学结论，而这一问题在过去曾困扰过其他类型的遗传标记。
