Lozano-Puentes Hair Santiago, Tarazona-Pulido Lina, López-Alvarez Diana, Ruiz-Sanchez Eduardo, Costa Geison M, Díaz-Ariza Lucia A
Grupo de Investigación en Agricultura Biológica, Departamento de Biología, Pontificia Universidad Javeriana, Sede Bogotá, Bogotá 110231, Colombia.
Grupo de Investigación Fitoquímica, Departamento de Química, Pontificia Universidad Javeriana, Sede Bogotá, Bogotá 110231, Colombia.
Int J Mol Sci. 2025 Jun 19;26(12):5879. doi: 10.3390/ijms26125879.
, a native bamboo species of ecological and economic importance, has been widely studied in Colombia. This study focused on evaluating the genetic diversity and population structure of from six natural populations in the Department of Nariño, Colombia, using restriction site-associated DNA sequencing (RADseq). A total of 224,996 high-quality SNPs were identified across 48 individuals. Observed heterozygosity (Ho) ranged from 0.398 in Consacá to 0.78 in Tumaco, while expected heterozygosity (He) was lower in all cases, ranging from 0.291 to 0.597. All populations exhibited negative inbreeding coefficients (FIS), from -0.316 to -0.763, indicating an excess of heterozygotes and suggesting predominantly outcrossing reproduction. Analysis of molecular variance (AMOVA) revealed that most genetic variation resides within individuals (92.54%), with low differentiation among populations (7.46%). Population structure and phylogenetic analyses identified two main genetic clusters, likely reflecting the origin of the planting material. Our results revealed that chromosomes CM070500.1, CM070502.1, CM070503.1, CM070504.1, CM070508.1, and CM070510.1 exhibited the highest SNP density, suggesting the presence of genomic regions with elevated variability. In contrast, chromosomes with lower SNP density suggested conservated genes related to Flavone Synthase II. This study is the first to evaluate genetic materials from the Department of Nariño. These findings highlight the significant genetic diversity in and the density of SNPs, and provide suggestions for conservation planning and the development of targeted breeding programs for this non-model tropical species.
作为一种具有生态和经济重要性的本土竹种，已在哥伦比亚得到广泛研究。本研究聚焦于利用限制性位点关联DNA测序（RADseq）评估哥伦比亚纳里尼奥省六个自然种群的[竹种名称]的遗传多样性和种群结构。在48个个体中总共鉴定出224,996个高质量单核苷酸多态性（SNP）。观察到的杂合度（Ho）在康萨卡为0.398，在图马科为0.78，而预期杂合度（He）在所有情况下都较低，范围从0.291到0.597。所有种群的近交系数（FIS）均为负值，从 -0.316到 -0.763，表明杂合子过剩，提示主要为异交繁殖。分子方差分析（AMOVA）显示，大多数遗传变异存在于个体内（92.54%），种群间分化程度较低（7.46%）。种群结构和系统发育分析确定了两个主要的遗传簇，可能反映了种植材料的来源。我们的结果显示，染色体CM070500.1、CM070502.1、CM070503.1、CM070504.1、CM070508.1和CM070510.1表现出最高的SNP密度，表明存在变异率升高的基因组区域。相比之下，SNP密度较低的染色体提示与黄酮合酶II相关的保守基因。本研究首次评估了纳里尼奥省的遗传材料。这些发现突出了[竹种名称]显著的遗传多样性和SNP密度，并为这种非模式热带物种的保护规划和定向育种计划的制定提供了建议。
