Shokouhfard Maliheh, Kermanshahi Rouha Kasra, Shahandashti Roya Vahedi, Feizabadi Mohammad Mehdi, Teimourian Shahram
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2015 Oct;18(10):1001-7.
Serratia marcescens is one of the nosocomial pathogen with the ability to form biofilm which is an important feature in the pathogenesis of S. marcescens. The aim of this study was to determine the anti-adhesive properties of a biosurfactant isolated from Lactobacillus acidophilus ATCC 4356, on S. marcescens strains.
Lactobacillus acidophilus ATCC 4356 was selected as a probiotic strain for biosurfactant production. Anti-adhesive activities was determined by pre-coating and co- incubating methods in 96-well culture plates.
The FTIR analysis of derived biosurfactant revealed the composition as protein component. Due to the release of such biosurfactants, L. acidophilus was able to interfere with the adhesion and biofilm formation of the S. marcescens strains. In co-incubation method, this biosurfactant in 2.5 mg/ml concentration showed anti-adhesive activity against all tested strains of S. marcescens (P<0.05).
Our results show that the anti-adhesive properties of L. acidophilus biosurfactant has the potential to be used against microorganisms responsible for infections in the urinary, vaginal and gastrointestinal tracts, as well as skin, making it a suitable alternative to conventional antibiotics.
粘质沙雷氏菌是一种能够形成生物膜的医院病原体，生物膜是粘质沙雷氏菌发病机制中的一个重要特征。本研究的目的是确定从嗜酸乳杆菌ATCC 4356中分离出的一种生物表面活性剂对粘质沙雷氏菌菌株的抗粘附特性。
选择嗜酸乳杆菌ATCC 4356作为生产生物表面活性剂的益生菌菌株。通过在96孔培养板中预包被和共孵育方法测定抗粘附活性。
对衍生生物表面活性剂的傅里叶变换红外光谱分析表明其成分是蛋白质成分。由于此类生物表面活性剂的释放，嗜酸乳杆菌能够干扰粘质沙雷氏菌菌株的粘附和生物膜形成。在共孵育方法中，这种浓度为2.5 mg/ml的生物表面活性剂对所有测试的粘质沙雷氏菌菌株均显示出抗粘附活性（P<0.05）。
我们的结果表明，嗜酸乳杆菌生物表面活性剂的抗粘附特性有可能用于对抗引起泌尿道、阴道、胃肠道以及皮肤感染的微生物，使其成为传统抗生素的合适替代品。
