Randell Rachel L, Gulati Ajay S, Cook Suzanne F, Martin Christopher F, Chen Wenli, Jaeger Elizabeth L, Schoenborn Alexi A, Basta Patricia V, Dejong Hendrik, Luo Jingchun, Gallant Marisa, Sandler Robert S, Long Millie D, Kappelman Michael D
Department of Pediatrics, Duke University School of Medicine, Duke University, Durham, NC, United States.
JMIR Res Protoc. 2016 Jan 5;5(1):e3. doi: 10.2196/resprot.5171.
The Internet has successfully been used for patient-oriented survey research. Internet-based translational research may also be possible.
Our aim was to study the feasibility of collecting biospecimens from CCFA Partners, an Internet-based inflammatory bowel disease (IBD) cohort.
From August 20, 2013, to January 4, 2014, we randomly sampled 412 participants, plus 179 from a prior validation study, and invited them to contribute a biospecimen. Participants were randomized to type (blood, saliva), incentive (none, US $20, or US $50), and collection method for blood. The first 82 contributors were also invited to contribute stool. We used descriptive statistics and t tests for comparisons.
Of the 591 participants, 239 (40.4%) indicated interest and 171 (28.9%) contributed a biospecimen. Validation study participants were more likely to contribute than randomly selected participants (44% versus 23%, P<.001). The return rate for saliva was higher than blood collected by mobile phlebotomist and at doctors' offices (38%, 31%, and 17% respectively, P<.001). For saliva, incentives were associated with higher return rates (43-44% versus 26%, P=.04); 61% contributed stool. Fourteen IBD-associated single nucleotide polymorphisms were genotyped, and risk allele frequencies were comparable to other large IBD populations. Bacterial DNA was successfully extracted from stool samples and was of sufficient quality to permit quantitative polymerase chain reaction for total bacteria.
Participants are willing to contribute and it is feasible to collect biospecimens from an Internet-based IBD cohort. Home saliva kits yielded the highest return rate, though mobile phlebotomy was also effective. All samples were sufficient for genetic testing. These data support the feasibility of developing a centralized collection of biospecimens from this cohort to facilitate IBD translational studies.
互联网已成功应用于以患者为导向的调查研究。基于互联网的转化研究也或许可行。
我们旨在研究从CCFA Partners（一个基于互联网的炎症性肠病（IBD）队列）收集生物样本的可行性。
从2013年8月20日至2014年1月4日，我们随机抽取了412名参与者，外加来自先前验证研究的179名参与者，并邀请他们提供生物样本。参与者被随机分配样本类型（血液、唾液）、激励措施（无、20美元或50美元）以及血液采集方法。前82名提供样本者还被邀请提供粪便样本。我们使用描述性统计和t检验进行比较。
在591名参与者中，239名（40.4%）表示有兴趣，171名（28.9%）提供了生物样本。验证研究的参与者比随机选择的参与者更有可能提供样本（44%对23%，P<0.001）。唾液的回收率高于由流动采血员和在医生办公室采集的血液（分别为38%、31%和17%，P<0.001）。对于唾液，激励措施与更高的回收率相关（43 - 44%对26%，P = 0.04）；61%的人提供了粪便样本。对14个与IBD相关的单核苷酸多态性进行了基因分型，风险等位基因频率与其他大型IBD人群相当。细菌DNA成功从粪便样本中提取出来，并且质量足以允许对总细菌进行定量聚合酶链反应。
参与者愿意提供样本，从基于互联网的IBD队列中收集生物样本是可行的。家用唾液采集试剂盒的回收率最高，不过流动采血也很有效。所有样本都足以进行基因检测。这些数据支持从该队列集中收集生物样本以促进IBD转化研究的可行性。
