霍乱弧菌肠毒素A-B+基因在大肠杆菌中的表达
The expression of enterotoxin A-B+ gene of V. cholerae in E. coli.
作者信息
Ma Q J, Zhou J G, Yu X Q, Liu C X, Xiong L S, Xu Y Q, Huang C F
机构信息
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
出版信息
Sci China B. 1989 Feb;32(2):186-92.
PMID:2673288
Abstract
The cloning in E. coli of a cholerae toxin gene that is A-B+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.
摘要
通过DNA重组技术已成功构建出一种A-B+型霍乱毒素基因在大肠杆菌中的克隆体。携带重组质粒pMM-CTB的大肠杆菌细胞已被证明能产生大量作为细胞外蛋白分泌的CTB亚基。
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