Evdokimova N M, Zakharenko V I, Aleshkin G I, Andreeva I V, Skavronskaia A G
Genetika. 1987 Sep;23(9):1581-7.
The recombinant plasmid RP4 omega elt carrying Escherichia coli heat-labile enterotoxin elt genes with 70-80% homology with genes vct of Vibrio cholerae has been constructed. We used this plasmid to determine localization of the cholerae toxin genes vct on the map of Vibrio cholerae cholerae. Two types of the donors were revealed in matings of 10 strains of V. cholerae cholerae 569B/RP4 omega elt with the polyauxotrophic recipients RV31 and RV175: some strains had enhanced frequency of mobilization of ilv-1 and lys-6 markers, the others--of trp-1. Our data suggest that structural vct genes are located within two regions of V. cholerae cholerae 569B chromosome: trp-1 and ilv-1--lys-6.
已构建携带与霍乱弧菌vct基因具有70 - 80%同源性的大肠杆菌不耐热肠毒素elt基因的重组质粒RP4 omega elt。我们用该质粒确定霍乱毒素基因vct在霍乱弧菌图谱上的定位。在10株霍乱弧菌569B/RP4 omega elt与多营养缺陷型受体RV31和RV175的交配中发现了两种类型的供体：一些菌株中ilv - 1和lys - 6标记的转移频率增加，另一些菌株中trp - 1标记的转移频率增加。我们的数据表明，vct结构基因位于霍乱弧菌569B染色体的两个区域：trp - 1和ilv - 1 - lys - 6。
