Ehrlich Marite, Ivask Marilin, Raasmaja Atso, Kõks Sulev
Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia
Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia.
Physiol Rep. 2016 Jan;4(1). doi: 10.14814/phy2.12660.
In this study, we investigated the physiological regulation of energy metabolism in wild-type (WT) and WFS1-deficient (Wfs1KO) mice by measuring the effects of menthol treatment on the O2 consumption, CO2 production, rectal body temperature, and heat production. The basal metabolism and behavior was different between these genotypes as well as TRP family gene expressions. Wfs1KO mice had a shorter life span and weighed less than WT mice. The food and water intake of Wfs1KO mice was lower as well as the body temperature when compared to their WT littermates. Furthermore, Wfs1KO mice had higher basal O2 consumption, and CO2 and heat production than WT mice. In addition, Wfs1KO mice showed a higher response to menthol administration in comparison to WT mice. The strongest menthol effect was seen on different physiological measures 12 h after oral administration. The highest metabolic response of Wfs1KO mice was seen at the menthol dose of 10 mg/kg. Menthol increased O2 consumption, and CO2 and heat production in Wfs1KO mice when compared to their WT littermates. In addition, the expression of Trpm8 gene was increased. In conclusion, our results show that the Wfs1KO mice develop a metabolic phenotype characterized with several physiological dysfunctions.
在本研究中，我们通过测量薄荷醇处理对氧气消耗、二氧化碳产生、直肠体温和产热的影响，研究了野生型（WT）和WFS1缺陷型（Wfs1KO）小鼠能量代谢的生理调节。这些基因型之间的基础代谢和行为以及瞬时受体电位（TRP）家族基因表达存在差异。Wfs1KO小鼠的寿命较短，体重比WT小鼠轻。与同窝野生型小鼠相比，Wfs1KO小鼠的食物和水摄入量以及体温较低。此外，Wfs1KO小鼠的基础氧气消耗量、二氧化碳和产热比WT小鼠高。此外，与WT小鼠相比，Wfs1KO小鼠对薄荷醇给药的反应更高。口服给药12小时后，在不同生理指标上观察到最强的薄荷醇效应。Wfs1KO小鼠在薄荷醇剂量为10mg/kg时出现最高的代谢反应。与同窝野生型小鼠相比，薄荷醇增加了Wfs1KO小鼠的氧气消耗、二氧化碳和产热。此外，Trpm8基因的表达增加。总之，我们的结果表明，Wfs1KO小鼠表现出一种具有多种生理功能障碍特征的代谢表型。
