Wei Yu-Rong, Yang Xue-Yun, Li Yuan-Guo, Wei Jie, Ma Wen-Ge, Ren Zhi-Guang, Guo Hui-Ling, Wang Tie-Cheng, Mi Xiao-Yun, Adili Gulizhati, Miao Shu-Kui, Shaha Ayiqiaolifan, Gao Yu-Wei, Huang Jiong, Xia Xian-Zhu
College of Animal Science and Technology, Shihezi University, Shihezi, 832003, China.
Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi, 830000, China.
Arch Virol. 2016 Apr;161(4):867-72. doi: 10.1007/s00705-015-2724-4. Epub 2016 Jan 5.
We conducted a serological survey to detect antibodies against avian influenza virus (AIV) in Gazella subgutturosa, Canis lupus, Capreolus pygargus, Sus scrofa, Cervus elaphus, Capra ibex, Ovis ammon, Bos grunniens and Pseudois nayaur in Xinjiang, China. Two hundred forty-six sera collected from 2009 to 2013 were assayed for antibodies against H5, H7 and H9 AIVs using hemagglutination inhibition (HI) tests and a pan-influenza competitive ELISA. Across all tested wildlife species, 4.47 % harbored anti-AIV antibodies that were detected by the HI assay. The seroprevalence for each AIV subtype across all species evaluated was 0 % for H5 AIV, 0.81 % for H7 AIV, and 3.66 % for H9 AIV. H7-reactive antibodies were found in Canis lupus (9.09 %) and Ovis ammon (4.55 %). H9-reactive antibodies were found in Gazella subgutturosa (4.55 %), Canis lupus (27.27 %), Pseudois nayaur (23.08 %), and Ovis ammon (4.55 %). The pan-influenza competitive ELISA results closely corresponded to the cumulative prevalence of AIV exposure as measured by subtype-specific HI assays, suggesting that H7 and H9 AIV subtypes predominate in the wildlife species evaluated. These data provide evidence of prior infection with H7 and H9 AIVs in non-avian wildlife in Xinjiang, China.
我们开展了一项血清学调查，以检测中国新疆地区鹅喉羚、狼、斑羚、野猪、马鹿、北山羊、盘羊、牦牛和岩羊体内针对禽流感病毒（AIV）的抗体。使用血凝抑制（HI）试验和全流感竞争ELISA，对2009年至2013年采集的246份血清进行了H5、H7和H9亚型禽流感病毒抗体检测。在所有检测的野生动物物种中，4.47%的个体通过HI试验检测出携带抗禽流感病毒抗体。在所有评估物种中，H5亚型禽流感病毒的血清阳性率为0%，H7亚型禽流感病毒为0.81%，H9亚型禽流感病毒为3.66%。在狼（9.09%）和盘羊（4.55%）中发现了H7反应性抗体。在鹅喉羚（4.55%）、狼（27.27%）、岩羊（23.08%）和盘羊（4.55%）中发现了H9反应性抗体。全流感竞争ELISA结果与通过亚型特异性HI试验测得的禽流感病毒暴露累积患病率密切相关，表明H7和H9亚型禽流感病毒在评估的野生动物物种中占主导地位。这些数据为中国新疆非禽类野生动物先前感染H7和H9亚型禽流感病毒提供了证据。
