Donohue J, Bailey M, Gray R, Holen J, Huang T M, Keevan J, Mattimiro C, Putterman C, Stalder A, Defreese J
Diagnostic Division, Abbott Laboratories, Abbott Park, IL 60064.
Clin Chem. 1989 Sep;35(9):1874-7.
An immunoassay system based on enzyme immunoassay technology has been developed for quantitative panel testing. The system includes test card disposables, reagents, and an instrument. Patients' samples are processed semiautomatically in the instrument with minimum user intervention. The test card has multiple test areas at individual locations on a membrane solid phase so that simultaneous determinations from a single specimen are possible. Each panel also includes positive and negative reagent procedural controls. Factory-determined calibration curves for each analyte are provided in barcode form with each test kit. The reagents include a specimen dilution buffer, enzyme conjugate, and precipitogenic substrate. Up to 10 test cards at a time can be processed in random-access and continuous-access modes, with automated agitation of sample and reagents over the solid phase, temperature-controlled incubation, and membrane washing and reading, data reduction, and printout of results. The optical reader measures diffuse reflectance and features source intensity and wavelength compensation.
已开发出一种基于酶免疫测定技术的免疫测定系统，用于定量组合检测。该系统包括一次性测试卡、试剂和一台仪器。患者样本在仪器中进行半自动处理，用户干预最少。测试卡在膜固相的各个位置有多个测试区域，因此可以从单个样本中同时进行测定。每个检测组合还包括阳性和阴性试剂程序对照。每个试剂盒都以条形码形式提供每种分析物的工厂确定校准曲线。试剂包括样本稀释缓冲液、酶结合物和沉淀性底物。一次最多可处理10张测试卡，采用随机存取和连续存取模式，对样本和试剂在固相上进行自动搅拌、温度控制孵育、膜洗涤和读数、数据处理以及结果打印输出。光学阅读器测量漫反射率，并具有光源强度和波长补偿功能。
