采用第二代酶免疫测定法对丙型肝炎病毒反应性血清进行研究。
Investigation of sera reactive to hepatitis C virus by second-generation enzyme immunoassay.
作者信息
Frost E H
机构信息
Department of Microbiology, Centre Hospitalier Universitaire de Sherbrooke, Quebec, Canada.
出版信息
J Clin Microbiol. 1993 Jan;31(1):163-4. doi: 10.1128/jcm.31.1.163-164.1993.
Abstract
Hepatitis C virus antibodies were detected by a second-generation enzyme immunoassay and investigated with a second-generation recombinant immunoblot assay. Most sera with optical density values of > or = 2.0, particularly those with optical densities of > or = 2.0 after a further 10-fold dilution, were positive in the second-generation recombinant immunoblot assay. Repeat testing of hemodialysis patients revealed excellent reproducibility and increased sensitivity of the second-generation enzyme immunoassay.
摘要
采用第二代酶免疫测定法检测丙型肝炎病毒抗体，并通过第二代重组免疫印迹试验进行研究。大多数光密度值≥2.0的血清，尤其是那些在进一步10倍稀释后光密度≥2.0的血清，在第二代重组免疫印迹试验中呈阳性。对血液透析患者的重复检测显示第二代酶免疫测定法具有出色的可重复性和更高的灵敏度。
相似文献
1
Investigation of sera reactive to hepatitis C virus by second-generation enzyme immunoassay.采用第二代酶免疫测定法对丙型肝炎病毒反应性血清进行研究。
J Clin Microbiol. 1993 Jan;31(1):163-4. doi: 10.1128/jcm.31.1.163-164.1993.
2
Enzyme immunoassays for anti-hepatitis C virus antibodies improved specificity and analytical sensitivity by combination of three different recombinant viral proteins in second generation tests.第二代检测中通过组合三种不同的重组病毒蛋白，丙型肝炎病毒抗体酶免疫测定提高了特异性和分析灵敏度。
Eur J Clin Chem Clin Biochem. 1992 Jul;30(7):397-404. doi: 10.1515/cclm.1992.30.7.397.
3
Evaluation of third-generation screening and confirmatory assays for HCV antibodies.丙型肝炎病毒抗体第三代筛查和确证检测方法的评估
Vox Sang. 1994;66(2):122-9. doi: 10.1111/j.1423-0410.1994.tb00293.x.
4
Evaluation of third-generation assays for detection of anti-hepatitis C virus (HCV) antibodies and comparison with presence of HCV RNA in blood donors reactive to c100-3 antigen.用于检测抗丙型肝炎病毒（HCV）抗体的第三代检测方法的评估以及与对c100-3抗原呈反应性的献血者血液中HCV RNA存在情况的比较。
J Clin Microbiol. 1994 Sep;32(9):2272-5. doi: 10.1128/jcm.32.9.2272-2275.1994.
5
Enhanced detection of antibodies to hepatitis C virus by use of a third-generation recombinant immunoblot assay.使用第三代重组免疫印迹法增强丙型肝炎病毒抗体检测
J Med Virol. 1994 Jul;43(3):259-61. doi: 10.1002/jmv.1890430312.
6
High specificity of hepatitis C second-generation enzyme immunoassay in HIV-infected patients.
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 May 1;9(1):97-8. doi: 10.1097/00042560-199505010-00014.
7
Improved diagnosis of chronic hepatitis C virus infection by detection of antibody to multiple epitopes: confirmation by antibody to synthetic oligopeptides.通过检测针对多个表位的抗体改进慢性丙型肝炎病毒感染的诊断：合成寡肽抗体的验证
J Med Virol. 1992 Nov;38(3):167-71. doi: 10.1002/jmv.1890380303.
8
Second generation hepatitis C virus assays: performance when testing African sera.第二代丙型肝炎病毒检测方法：检测非洲血清时的性能
J Med Virol. 1993 Sep;41(1):35-8. doi: 10.1002/jmv.1890410108.
9
Hepatitis C virus infection in kidney transplant recipients.肾移植受者中的丙型肝炎病毒感染
Hepatology. 1993 Nov;18(5):1027-31. doi: 10.1016/0270-9139(93)90452-s.
10
Evaluation of first- and second-generation RIBA kits for detection of antibody to hepatitis C virus.第一代和第二代重组免疫印迹法（RIBA）试剂盒检测丙型肝炎病毒抗体的评估
J Clin Microbiol. 1991 Oct;29(10):2329-30. doi: 10.1128/jcm.29.10.2329-2330.1991.
本文引用的文献
1
Enzyme immunoassay system for panel testing.用于组合检测的酶免疫分析系统。
Clin Chem. 1989 Sep;35(9):1874-7.
2
Hepatitis C virus antibodies among risk groups in Spain.西班牙风险人群中的丙型肝炎病毒抗体。
Lancet. 1989 Aug 5;2(8658):294-7. doi: 10.1016/s0140-6736(89)90485-6.
3
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.从经血传播的非甲非乙型病毒性肝炎基因组中分离出一个cDNA克隆。
Science. 1989 Apr 21;244(4902):359-62. doi: 10.1126/science.2523562.
4
Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis.前瞻性随访的急性和慢性非甲非乙型肝炎输血受者中丙型肝炎病毒抗体的检测
N Engl J Med. 1989 Nov 30;321(22):1494-500. doi: 10.1056/NEJM198911303212202.
5
An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.一种针对人类非甲非乙型肝炎主要致病病毒的循环抗体检测方法。
Science. 1989 Apr 21;244(4902):362-4. doi: 10.1126/science.2496467.
6
Early US experience with anti-HCV kit in blood donors.美国早期在献血者中使用抗丙型肝炎病毒检测试剂盒的经验。
Lancet. 1990 Jul 28;336(8709):244-5. doi: 10.1016/0140-6736(90)91767-5.
7
Recombinant immunoblot assay for hepatitis C virus antibody as predictor of infectivity.
Lancet. 1990 Apr 21;335(8695):982-3. doi: 10.1016/0140-6736(90)91055-f.
8
Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result?慢性活动性肝炎中的丙型肝炎病毒抗体：致病因素还是假阳性结果？
Lancet. 1990 Mar 31;335(8692):754-7. doi: 10.1016/0140-6736(90)90870-b.
9
Evaluation of hepatitis C virus kits.丙型肝炎病毒检测试剂盒的评估
J Clin Microbiol. 1991 Nov;29(11):2616-7. doi: 10.1128/jcm.29.11.2616-2617.1991.
10
Detection of antibodies to hepatitis C virus in U.S. blood donors.美国献血者中丙型肝炎病毒抗体的检测
J Clin Microbiol. 1991 Mar;29(3):551-6. doi: 10.1128/jcm.29.3.551-556.1991.
Zotero 插件