用于光遗传学和生物成像应用的染料敏化核/活性壳上转换纳米颗粒
Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications.
作者信息
Wu Xiang, Zhang Yuanwei, Takle Kendra, Bilsel Osman, Li Zhanjun, Lee Hyungseok, Zhang Zijiao, Li Dongsheng, Fan Wei, Duan Chunying, Chan Emory M, Lois Carlos, Xiang Yang, Han Gang
机构信息
State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian, 116012, People's Republic of China.
Department of Materials Science & Engineering, Zhejiang University , Hangzhou, 310027, People's Republic of China.
出版信息
ACS Nano. 2016 Jan 26;10(1):1060-6. doi: 10.1021/acsnano.5b06383. Epub 2016 Jan 11.
Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb(3+)) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.
近红外（NIR）染料敏化上转换纳米粒子（UCNPs）可以拓宽UCNPs的吸收范围并提高其上转换效率。在此，我们通过在UCNP壳层中掺杂镱离子（Yb(3+)），在染料敏化的核/活性壳UCNPs中实现了显著增强的上转换发光，这架起了从染料到UCNP核的能量转移桥梁。结果，我们协同了两种最实用的上转换增强效应器（染料敏化和核/壳增强）来放大上转换效率。我们展示了使用这些UCNPs的两种生物医学应用。通过使用染料敏化的核/活性壳UCNP嵌入聚（甲基丙烯酸甲酯）聚合物可植入系统，我们成功地将光遗传学神经元激发窗口转移到生物相容性好且能穿透深层组织的800 nm波长。此外，UCNPs用Pluronic F127进行了水溶性处理，具有高上转换效率，并且可以在小鼠模型中成像。
Zotero 插件