Radziejewska-Lebrecht J, Mayer H
Instytut Mikrobiologii, Uniwersytet Lódzki.
Eur J Biochem. 1989 Aug 15;183(3):573-81. doi: 10.1111/j.1432-1033.1989.tb21086.x.
The complete core structure present in the lipopolysaccharide of the R mutant R110/1959 from Proteus mirabilis (Proteus type II core) was investigated using methylation analysis and a number of degradation methods such as Smith degradation and beta-elimination. These studies combined with earlier work on a Rc-type mutant of P. mirabilis O28 (R4/O28) which shares the same inner core region, allowed formulation of the complete core structure of the Proteus type II core as shown in Scheme 1. (formula; see text) A characteristic feature of the Proteus core of type II is the presence of two units of D-galacturonic acid (DGalA); one in terminal, the other one in a chain-linked position. In addition, the presence of the two isomers of glycero-D-manno-heptose (LDHep and DDHep) and the lack of galactose are conspicuous. DDHep occupies a terminal position in the external core region, whereas the three units of LDHep in addition to dOclA form, as in other enterobacterial core types, the internal core region. The taxonomic significance of the presence of DGalA in the Proteus type II core, but also in all R cores of other Proteeae investigated so far, will be discussed.
利用甲基化分析以及诸如史密斯降解和β-消除等多种降解方法，对奇异变形杆菌（变形杆菌II型核心）的R突变体R110/1959脂多糖中存在的完整核心结构进行了研究。这些研究与早期对奇异变形杆菌O28（R4/O28）的Rc型突变体的研究相结合，该突变体与R110/1959共享相同的内核区域，从而得以确定变形杆菌II型核心的完整核心结构，如图1所示。（分子式；见正文）II型变形杆菌核心的一个特征是存在两个D-半乳糖醛酸（DGalA）单元；一个位于末端，另一个处于链连接位置。此外，甘油-D-甘露庚糖的两种异构体（LDHep和DDHep）的存在以及半乳糖的缺失也很明显。DDHep占据外核心区域的末端位置，而除了dOclA之外的三个LDHep单元与其他肠杆菌核心类型一样，构成内核心区域。将讨论DGalA在变形杆菌II型核心中以及在迄今为止所研究的其他变形菌科所有R核心中存在的分类学意义。
